了哥王叶绿体基因组分析OA北大核心CSTPCD

[Objective]This study is aimed to clarify the structure traits of chloroplast genome and phylogenetic status of medicinal plant Wikstroemia indica,providing a scientific basis for the resource protection and sustainable utilization of W.indica.[Method]An Illumina high-throughput sequencing platform was employed to acquire the chloroplast genome sequence which was then assembled,annotated,compared and analyzed using bioinformatics technologies and software.[Result]The chloroplast genome of W.indica was an annular quadripartite molecule with a length of 149 864 bp and composed of a large single copy(LSC,86 347 bp),a small single copy(SSC,10 601 bp)and a couple of inverted repeat(IR,26 458 bp)and 124 genes were annotated in the chloroplast genome.In the chloroplast genome there were a total of 24 180 codon,which were divided into 64 types of which 30 types of codon were used frequently and 29 of them ended with A/T.Altogether 93 chloroplast simple sequence repeat(SSR)were identified from the chloroplast genome with Mononucleotide being the predominate type taking up a number of 72 and the motifs formed by A/T contributing the most and obvious variations were observed in IR boundary between W.indica and closely related plants whereas sequence alignment and phylogenetic tree revealed that W.indica shared high sequence identity with W.nutans.[Conclusion]The chloroplast genome of W.indica exhibited typical structure of plant chloroplast genome,and possessed codon preference and several SSRs with rich polymorphism.Additionally,the chloroplast genome of W.indicashowed the closest similarity to W.nutans.[Ch,5 fig.2 tab.24 ref.]