中国兽医科学2024，Vol.54Issue(3)：300-307,8.DOI:10.16656/j.issn.1673-4696.2024.0033
W/O/W佐剂添加两种免疫增强剂对口蹄疫灭活疫苗的影响
Effect of W/O/W adjuvant added with two immunopotentiator on inactivated foot-and-mouth disease vaccine
摘要
Abstract
To enhance the immune efficacy of the inactivated foot-and-mouth disease vaccine,this study incorporated immunopotentiators into the water-in-oil-in-water(W/O/W)adjuvant formulation.Two types of immunopotentiators,natural compound vitamin E(VE)and synthetic derivative imiquimod(IMQ-Chol)were used.The basic formulation was mixed with the inactivated foot-and-mouth disease virus,along with three different doses(low,medium,high)of each immunopotentiator,to prepare various adjuvants.The appearance and physicochemical properties of each vaccine were determined,including stability,viscosity,particle size,and polydispersity index(PDI).The safety of the immunopotentiator was evaluated through mouse body weight,routine blood test,blood biochemistry,and histological changes.Furthermore,the impact of the immunopotentiator on vaccine immune efficacy was assessed by examining specific antibodies,antibody subtypes,cytokine levels in mouse sera,and differentiation of mouse splenic T lymphocytes.The results showed that the prepared vaccines were milky white W/O/W emul-sions with no phase separation after 9 weeks at 37 ℃.The viscosity remained below 16 cp,and the parti-cle size ranged from 270 nm to 370 nm,with the PDI between 0.34 and 0.42.None of the immunopotentiators affected mouse body weight(P＞0.05),and routine blood test,blood biochemistry and histological sections indicated that both immunopotentiators had good biological safety.Compared to the basic formula group,the groups immunized with VE or IMQ-Chol showed significantly higher level of specific antibody(P＜0.01).Notably,the group with high-dose VE and low-dose IMQ-Chol significantly increased the spe-cific antibody level(P＜0.001),as well as the secretion levels of Th1-related molecules(IgG2a,IFN-γ,IL-12)and Th2-related molecules(IgG1,IL-4,IL-6)(P＜0.05).The proportions of CD3+CD4+T cells in these two groups were 75.2％and 77.3％,which were higher than the 69.6％in the basic formulation group,and the CD4+/CD8+ratio was higher as well(P＜0.05).The above results indicated that both VE and IMQ-Chol did not impact the physicochemical properties of the vaccine,maintained good safety,and the addition of high-dose VE or low-dose IMQ-Chol could enhance the immune efficacy of the inactivated foot-and-mouth disease vaccine.关键词
口蹄疫/W/O/W佐剂/免疫增强剂/维生素E/咪喹莫特Key words
foot-and-mouth disease/W/O/W adjuvant/immunopotentiator/vitamin E/imiquimod分类
农业科技引用本文复制引用
周宇杰,卢宇,许泽玉,唐健中,钱莺娟,邓碧华..W/O/W佐剂添加两种免疫增强剂对口蹄疫灭活疫苗的影响[J].中国兽医科学,2024,54(3):300-307,8.基金项目
国家重点研发揭榜挂帅项目(2021YFD1801200) （2021YFD1801200）
江苏省农业科技自主创新资金项目[CX(22)1011] （22）
江苏省重点研发计划项目(BE2021351) （BE2021351）
