中国兽医科学2024，Vol.54Issue(3)：308-315,8.DOI:10.16656/j.issn.1673-4696.2024.0039
非洲马瘟病毒(AV1311株)核糖核酸标准物质的研制
Development of nucleic acid reference material for African horse sickness virus(strain AV1311)
摘要
Abstract
Reference materials(RMs)are the gold standard for molecular diagnosis technology.In view of the lack of African horse sickness virus(AHSV)RMs in China,AHSV(AV1311 strain)nucleic acid RM(NARM)was successfully prepared using AV1311 strain as raw material by virus culture,inactivation,packaging,and characteristic testing.At the same time,a digital PCR method was established to evalu-ate the homogeneity,stability,and quantification of the NARM in collaboration with 9 laboratories,and carried out clinical trials.The results of homogeneity evaluation showed there was no statistical differences within groups and between groups,and the samples were homogeneous.The stability investi-gation revealed that the NARM could be stored at-20 ℃ for 6 months,4 ℃ for 7 days,and at 25 ℃ for 24 hours.It could undergo up to 5 freeze-thaw cycles and requires transport on dry ice.The certified value of the NARM was(6.9±1.1)X 103 copies/μL.Clinical trials demonstrated that the prepared NARM had good interoperability with clinical samples.In conclusion,the prepared NARM has accurate quantification,good homogeneity and stable values,which had been successfully evaluated by National Administrative Committee[GBW(E)091272]and provides a material basis for the prevention of AHS in China.关键词
非洲马瘟病毒/核糖核酸标准物质/检测/定值/数字PCRKey words
African horse sickness virus/nucleic acid reference material/detection/quantifica-tion/digital PCR分类
农业科技引用本文复制引用
苏佳,张敏,白洪旭,张兵,赵炜,薛青红,杨承槐,陈晓春..非洲马瘟病毒(AV1311株)核糖核酸标准物质的研制[J].中国兽医科学,2024,54(3):308-315,8.基金项目
国家重点研发计划项目(2021YFD1800500) （2021YFD1800500）
