植物学报2024，Vol.59Issue(1)：34-53,20.DOI:10.11983/CBB23004
棉属光敏色素PHY基因家族的全基因组鉴定与驯化选择分析
Genome-wide Identification and Domestication Analysis of the Phytochrome PHY Gene Family in Gossypium
摘要
Abstract
Phytochrome is an important receptor for red and far-red light sensing in plants,and it plays a vital role in regulating the plant flowering period,improving crop yield potential and regulating plant stress resistance.Identification of PHY family genes in Gossypium,exploration of the patterns of inheritance and regulatory network of domestication and improvement,and identification of the key phytochrome genes in Gossypium,provides insights into the de novo domes-tication and breeding of early maturing Gossypium species.To identify the phytochrome genes of Gossypium,we used bioinformatics methods to analyze 5 phytochrome genes in Arabidopsis thaliana.Phylogenetic analysis showed that the PHY genes in Malvaceae species consisted of 4 subfamilies(PHYA,PHYB,PHYC and PHYE).Moreover,the domesti-cation selection analysis of PHY genes among different populations of G.hirsutum showed that the domestication process of PHY genes could be divided into two stages:domestication and improvement.Furthermore,the gene expression of the PHY gene family was analyzed using leaf RNA-sequencing data obtained from wild and cultivar genotypes of G.hirsutum under short-day(SD)and long-day(LD)conditions.The results showed that the expression of GhPHYA1Dt and GhPHYB1Dt were significantly different between SD and LD conditions.After 14 hours of long-day treatment,the ex-pression of GhPHYC1At and GHPHYE1At in the cultivar was significantly lower than that in wild species.These results lay a foundation for further study on domestication selection and functional mechanisms of Gossypium PHY genes and provide a theoretical basis for breeding new early maturing Gossypium varieties and de novo domestication.关键词
棉花/驯化/光敏色素/基因家族Key words
cotton/domestication/phytochrome/gene family引用本文复制引用
顾家琦,朱福慧,谢沛豪,孟庆营,郑颖,张献龙,袁道军..棉属光敏色素PHY基因家族的全基因组鉴定与驯化选择分析[J].植物学报,2024,59(1):34-53,20.基金项目
国家重点研发计划(No.2021YFF1000102)和华中农业大学中央高校基本科研项目(No.2021ZKPY013) （No.2021YFF1000102）
