食品科学技术学报2024，Vol.42Issue(2)：32-45,14.DOI:10.12301/spxb202300255
共递送姜黄素和EGCG脂质体的构建及其对神经炎症的作用
Construction of Co-Delivered Curcumin and EGCG Liposomes and Their Effects on Neuroinflammation
摘要
Abstract
To overcome the limitations of two bioactive components,curcumin and epigallocatechin gallate(EGCG),in terms of stability and metabolic kinetics,and to further exploit their synergistic antioxidant and anti-inflammatory effects,liposomes co-delivering curcumin and EGCG were constructed by thin-film sonication.The effects of phospholipid to cholesterol ratio,phospholipid to Tween 80 ratio,hydration time and sonication time on the liposomes were analysed using particle size,PDI and potential as evaluation indexes,and the liposomes were modified with lactoferrin and hyaluronic acid by electrostatic adsorption.A BV2 microglia inflammation model was constructed using lipopolysaccharide(LPS)to explore the interventional effects of liposomes modified with lactoferrin and hyaluronic acid for co-delivering of curcumin and EGCG on neuroinflammation.The results showed that liposomes co-delivering curcumin and EGCG in a uniform spherical shape with an average particle size of(131.53± 0.258)nm were successfully prepared at phospholipid to cholesterol ratio of 10∶1,phospholipid to Tween 80 ratio of 10∶3,hydration time of 40 min,and ultrasound time of 15 min.The liposomes were modified at a volume of 0.3 mL of lactoferrin and 0.5 mL of hyaluronic acid.The experimental results showed that the liposomes modified with lactoferrin and hyaluronic acid for co-delivery of curcumin and EGCG significantly improved the stability and antioxidant properties of curcumin and EGCG,inhibited LPS-induced alterations in cellular morphology,restored mitochondrial dysfunction,reduced intracellular reactive oxygen species production,and ameliorated the inflammatory effects of LPS-induced BV2 microglia.The results aimed to provide new ideas for further development of functional foods with neuroprotective effects.关键词
姜黄素/EGCG/脂质体/乳铁蛋白/神经炎症Key words
curcumin/EGCG/liposome/lactoferrin/neuroinflammation分类
轻工业引用本文复制引用
马德雪,闫晓佳,刘夫国,刘学波..共递送姜黄素和EGCG脂质体的构建及其对神经炎症的作用[J].食品科学技术学报,2024,42(2):32-45,14.基金项目
国家自然科学基金青年科学基金项目(21808187).National Natural Science Foundation of China Youth Science Fund Project(21808187). （21808187）
