食品科学技术学报2024，Vol.42Issue(2)：84-92,9.DOI:10.12301/spxb202300226
植物乳杆菌1-9代谢水苏糖机制研究
Study on Mechanism for Stachyose Metabolization by Lactiplantibacillus plantarum 1-9
摘要
Abstract
A strain of Lactobacillus was successfully isolated from fermented goat milk and identified as Lactiplantibacillus plantarum 1-9,which could efficiently utilize stachyose as the carbon source.In order to investigate the mechanism for stachyose metabolization by Lactiplantibacillus plantarum 1-9,the growth curve was established with stachyose as the only carbon source.The production of short chain fatty acids was monitored by high performance liquid chromatography,while thin-layer chromatography were used to monitor the metabolic procedures of stachyose by Lactiplantibacillus plantarum 1-9.Besides,on the basis of monitoring the gene expression of key stachyose metabolizing enzyme,the changes of related enzyme activity in fermentation broth were analyzed.The results showed that Lactiplantibacillus plantarum 1-9 metabolized stachyose and produced acetic acid and valeric acid as the major short chain fatty acids,which reached the highest concentration of 28.64 mg/mL and 2.74 mg/mL,respectively.The accumulation of short-chain fatty acids resulted in a significant decrease of pH to 4.00.Based on the results of metabolic procedure of stachyose and changes of gene expression and enzyme activity,gene 5-359 in the genome of Lactiplantibacillus plantarum 1-9 might encode α-galactosidase.This segment could hydrolyze the α-galactoside bond at the end of stachyose and produce galactose and small amount of lactose and sucrose,thus facilitating the utilization of stachyose by Lactiplantibacillus plantarum 1-9.This study aimed to provide theoretical reference for the reasonable design of symbiotics based on Lactiplantibacillus plantarum and stachyose,and the development of related products.关键词
植物乳杆菌1-9/水苏糖/代谢机制/α-半乳糖苷酶/基因组分析Key words
Lactiplantibacillus plantarum 1-9/stachyose/metabolic mechanism/α-galactosidase/genome analysis分类
轻工纺织引用本文复制引用
刘天仪,张文静,廖强,毛咏荷,刘军..植物乳杆菌1-9代谢水苏糖机制研究[J].食品科学技术学报,2024,42(2):84-92,9.基金项目
国家自然科学基金青年科学基金项目(31701549).National Natural Science Foundation of China Youth Science Foundation Project(31701549). （31701549）
