食品科学技术学报2024，Vol.42Issue(2)：109-119,11.DOI:10.12301/spxb202201049
青刺果抗氧化肽的分离纯化、结构鉴定及其分子对接解析
Isolation,Purification,Structure Identification and Molecular Docking Analysis of Antioxidant Peptide from Prinsepia utilis Royle
摘要
Abstract
Ultrafiltration and strong anion exchange chromatography were used to isolate and purify the protease hydrolysates of Prinsepia utilis Royle.The free radical scavenging rate of 1,1-diphenyl-2-picrylhydrazyl(DPPH)was used as the evaluation index to screen the peptide components with better antioxidant activity.The peptide sequence was identified by LC-MS/MS,and its physicochemical properties were analyzed by bioinformatics methods,and the secondary structure characteristics and docking sites with Keap1 protein were further analyzed by infrared spectroscopy and molecular docking technology.The results showed that the F-1 fractions had better antioxidant activity among the four component,and its DPPH free radical scavenging rate reached 27.67％.The peptide components with higher activity and molecular weight less than 1 kDa were selected,and the IC50 values of DPPH free radical scavenging of the three peptides(TALDVPPPR,TLSDAGVGGL,and DLINGGKDA)were 0.43,0.83,and 0.51 mg/mL,respectively,the antioxidant activity of TALDVPPPR was relatively high.Secondary structure analysis showed that the three antioxidant peptides were composed of α-helix,β-fold and β-turn,and the content of α-helix in TALDVPPPR was relatively low,making it easier to bind to free radicals.Molecular docking results showed that TALDVPPPR,TLSDAGVGGL and DLINGGKDA bound to Keapl protein by hydrogen bond and hydrophobic interaction,and released Nrf2 into the nucleus to activate the expression of a series of proteins with antioxidant and cell protective effects,that is,activate the Keap1-Nrf2/ARE pathway to play an antioxidant role.Therefore,TALDVPPPR,TLSDAGVGGL and DLINGGKDA could be used as new antioxidants.The purpose of this study was to provide theoretical reference for the development and utilization of functional peptides from Prinsepia utilis Royle.关键词
青刺果/抗氧化肽/二级结构/Keap1-Nrf2/ARE/分子对接Key words
Prinsepia utilis Royle/antioxidant peptide/secondary structure/Keap1-Nrf2/ARE/molecular docking分类
轻工纺织引用本文复制引用
于梦怡,刘世林,黄志远,董文明,范江平,王雪峰..青刺果抗氧化肽的分离纯化、结构鉴定及其分子对接解析[J].食品科学技术学报,2024,42(2):109-119,11.基金项目
国家自然科学基金地区科学基金项目(31960462) （31960462）
云南省农业联合专项重点项目(202101BD070001-013) （202101BD070001-013）
云南省科协第三届青年科技人才托举工程项目(2022-2024).National Natural Science Foundation of China Regional Projects(31960462) （2022-2024）
Yunnan Agricultural Joint Special Key Projects(202101BD070001-013) （202101BD070001-013）
The 3rd Young Elite Scientists Sponsorship Program by Yunnan Science and Technology Association(2022-2024). （2022-2024）
