大豆科学2024，Vol.43Issue(2)：139-150,12.DOI:10.11861/j.issn.1000-9841.2024.02.0139
大豆C2H2型锌指蛋白转录因子家族全基因组鉴定及表达分析
Genome-wide Identification and Expression Analysis of C2H2 Zinc Finger Protein Gene Family in Soybean
摘要
Abstract
In order to further search for potential candidate genes related to soybean yield,44 C2 H2 zinc finger protein family members from the genome database of soybean(Glycine max)were classified into 11 subfamilies by BLAST mapping of Arabidopsis thaliana L.C2 H2 zinc finger protein sequences using bioinformatics and transcriptomics technologies.The 44 C2H2 zinc finger protein family members screened from the soybean genome database were divided into 11 subfamilies,and analysed for phylogeny,conserved structural domains,cis-acting elements,sequencing data and transcriptome data.The results showed that the C2H2 zinc finger protein family members were distributed in 17 soybean chromosomes,and the structural domains of the genes in each subfamily were similar and highly conserved.The promoters of the members contained regulatory elements for stress and hormone resistance,and the RNA-seq results showed that eight genes were differentially expressed in different plant tissues.Quantitative real-time PCR analysis showed that three genes analysed were transcriptional regulators of the SUPERMAN,GmC1-1 iZFP42 was the corresponding gene in the Ln locus,which is involved in the regulation of soybean leaf shape and seed,GmC1-1iZFP12 and GmC1-1iZFP30 were transcriptional regulators of the SUPERMAN gene,and GmC1-1iZFP21 may be involved in the process of soybean grain formation and development,and is a candidate gene for regulating related processes.This study provides directions for further searching and mining of soybean high yield genes,as well as references and insights for the use of C2H2-type zinc finger proteins and bioinfonnatics in plants.关键词
大豆/C2H2/基因家族/表达分析Key words
soybean/C2H2/gene family/expression analysis引用本文复制引用
郭智滨,张亚坤,贺紫航,周强华,蔡占东,马启彬,年海..大豆C2H2型锌指蛋白转录因子家族全基因组鉴定及表达分析[J].大豆科学,2024,43(2):139-150,12.基金项目
国家重点研发计划"农业生物种质资源挖掘与创新利用"重点专项(2021YFD1201602) （2021YFD1201602）
广东省乡村振兴战略专项省级种业振兴行动项目(006939908/2022-00744) （006939908/2022-00744）
国家大豆产业技术体系建设项目(CARS-04-PS09) （CARS-04-PS09）
2021年大学生创新创业训练计划项目(202110564047). （202110564047）
