分析测试学报2024，Vol.43Issue(4)：566-573,8.DOI:10.12452/j.fxcsxb.23111301
氮掺杂碳量子点的制备及在细胞温度传感中的应用
Preparation and Application of Nitrogen-doped Carbon Quantum Dots in Cell Temperature Sensing
摘要
Abstract
In this study,nitrogen self-doped carbon quantum dots(N-CQDs)were prepared by mi-crowave method using biomass tomato as carbon and nitrogen sources.The morphology,structure,stability,photoelectric properties,temperature sensitivity and cytotoxicity were studied by field emission transmission electron microscope(TEM),X-ray photoelectron spectrometer(XPS)and fluo-rescence luminescence photometer,and were applied to temperature sensing in living cells.The ex-perimental results show that the prepared N-CQDs has strong stability,excellent photoelectric perfor-mance and strong sensitivity to temperature.In the temperature range of 25-65℃,the fluorescence intensity of N-CQDs decreases with the increase of temperature,and shows a good linear relation-ship.The N-CQDs can penetrate the cell membrane of 4T1 cells,showing low toxicity and biocom-patibility,and good temperature sensing fluorescence properties in 4T1 cells.It is indicated that the N-CQDs has great potential and value in the field of fluorescence temperature sensing in living cells,and is of great significance in the development of non-destructive cell measurement of nanoferters.It is expected to become a temperature-sensitive fluorescence nanoprobe and be widely used in biomedi-cal fields such as cell research.关键词
氮自掺杂碳量子点/微波法/光电性能/温敏性能/细胞温度传感Key words
nitrogen self-doped carbon quantum dots/microwave method/photoelectric property/temperature sensitive property/cell temperature sensing分类
化学化工引用本文复制引用
陈雨昕,胡月芳..氮掺杂碳量子点的制备及在细胞温度传感中的应用[J].分析测试学报,2024,43(4):566-573,8.基金项目
广西自然科学基金面上项目(2022GXNSFAA035469,2017GXNSFAA198274) （2022GXNSFAA035469,2017GXNSFAA198274）
贺州学院博士和教授科研启动基金项目(HZUBS202105,HZUJS202204) （HZUBS202105,HZUJS202204）
广西科技计划项目(桂科AD23023008) （桂科AD23023008）
