氮掺杂碳量子点的制备及在细胞温度传感中的应用OA北大核心CSTPCD

In this study,nitrogen self-doped carbon quantum dots(N-CQDs)were prepared by mi-crowave method using biomass tomato as carbon and nitrogen sources.The morphology,structure,stability,photoelectric properties,temperature sensitivity and cytotoxicity were studied by field emission transmission electron microscope(TEM),X-ray photoelectron spectrometer(XPS)and fluo-rescence luminescence photometer,and were applied to temperature sensing in living cells.The ex-perimental results show that the prepared N-CQDs has strong stability,excellent photoelectric perfor-mance and strong sensitivity to temperature.In the temperature range of 25-65℃,the fluorescence intensity of N-CQDs decreases with the increase of temperature,and shows a good linear relation-ship.The N-CQDs can penetrate the cell membrane of 4T1 cells,showing low toxicity and biocom-patibility,and good temperature sensing fluorescence properties in 4T1 cells.It is indicated that the N-CQDs has great potential and value in the field of fluorescence temperature sensing in living cells,and is of great significance in the development of non-destructive cell measurement of nanoferters.It is expected to become a temperature-sensitive fluorescence nanoprobe and be widely used in biomedi-cal fields such as cell research.