临床与病理杂志2024，Vol.44Issue(1)：1-7,7.DOI:10.11817/j.issn.2095-6959.2024.230169
外阴硬化性苔藓病变中的抗氧化酶变化
Changes of antioxidant enzymes in vulvar lichen sclerosus
摘要
Abstract
Objective:Vulvar lichen sclerosus(VLS)is an autoimmune chronic inflammatory disease characterized by itching and whitening of the vulvar skin.Currently,the pathogenesis of VLS remains unclear,although some studies suggest its association with oxidative stress.Antioxidant enzymes play a role in the elimination of peroxides.This study aims to investigate the expression of Mn superoxide dismutase(MnSOD),cytoplasmic peroxidase 2(PRDX2),and thioredoxin 1(thioredoxin 1)in VLS and their relationship with the occurrence and progression of VLS. Methods:A total of 40 VLS specimens(20 in the early stage and 20 in the advanced stage)were collected from the First Affiliated Hospital of China Medical University,and 20 normal vulvar skin tissue specimens were obtained from vulvar plastic surgery.The expressions of MnSOD,PRDX2,and TRX1 proteins in vulvar skin tissue were detected by the immunohistochemical SP method,and the average optical density value was measured under a high-power microscope.Pearson correlation analysis was used to calculate the correlation among MnSOD,PRDX2,and TRX1 in VLS tissues. Results:The average optical density value of MnSOD in VLS tissues was lower than that in normal vulvar skin tissues(P＜0.05),and the average optical density value of MnSOD in the early stage VLS tissues was higher than that in the advanced stage VLS tissues(P＜0.05).The average optical density value of PRDX2 in VLS tissues was higher than that in normal vulvar skin tissues(P＜0.05),and the average optical density value of PRDX2 in the early stage VLS tissues was lower than that in the advanced stage VLS tissues(P＜0.05).The average optical density value of TRX1 in VLS tissues was higher than that in normal vulvar skin tissues(P＜0.05),and the average optical density value of TRX1 in the early stage VLS tissues was lower than that in the advanced stage VLS tissues(P＜0.05).Pearson correlation analysis showed that the expression of MnSOD was negatively correlated with PRDX2(r=-0.615)and TRX1(r=-0.881)in VLS tissues(both P＜0.05),while the expression of PRDX2 and TRX1 in VLS tissues was positively correlated(r=0.670,P＜0.05). Conclusion:In the progression of VLS lesions,the expression of MnSOD decreases,while the expression of PRDX2 and TRX1 increases.关键词
外阴硬化性苔藓/超氧化物歧化酶/过氧化物酶2/硫氧还蛋白1Key words
vulvar lichen sclerosus/superoxide dismutase/peroxidase 2/thioredoxin 1引用本文复制引用
徐倩倩,王威,石洪爽,武昕..外阴硬化性苔藓病变中的抗氧化酶变化[J].临床与病理杂志,2024,44(1):1-7,7.基金项目
国家自然科学基金(30973190) （30973190）
辽宁省科学技术计划项目(2014021057).This work was supported by the National Natural Science Foundation(30973190)and the Science and Technology Plan Project of Liaoning Province(2014021057),China. （2014021057）
