现代食品科技2024，Vol.40Issue(3)：28-38,11.DOI:10.13982/j.mfst.1673-9078.2024.3.0330
具有抗氧化活性青稞酒糟蛋白酶解液对H2O2诱导HepG2细胞的保护作用
H2O2-induced Protective Effects of the Antioxidant Protease Hydrolysate of Highland Barley Brewer's Spent Grains on HepG2 Cells
摘要
Abstract
To illustrate the high-value utilization of highland barley brewer's spent grains,the process used to prepare its protease hydrolysate was optimized based on protein extraction from highland barley brewer's spent grains using the DPPH free radical scavenging rate and reducing power as indicators.Thereafter,the in vitro antioxidant activity and cytoprotective effects of the hydrolysates were examined.The soluble and white highland barley brewer's spent grains were found to be rich in protein,with protein contents of 31.46 g/100 g and 18.47 g/100 g,respectively.Using the ultrasound/cellulase-assisted alkali extraction and acid precipitation method,the protein extraction rates from the soluble and white highland barley brewer's spent grains were 76.10%(m/m)and 29.88%,respectively.Alkaline protease was found to be most suitable for enzymatic hydrolysis of the proteins of soluble highland barley brewer's grains.The optimal enzymatic hydrolysis conditions were as follows:solid to liquid ratio,1:60(m/V);ultrasonic treatment time,15 min;enzyme dosage,3.7%(m/m);and enzymatic hydrolysis time,3.5 h.Under these conditions,the DPPH free radical scavenging rate of the protease hydrolysate of soluble highland barley brewer's spent grains reached 95.02%.Different concentrations of the protease hydrolysate of soluble highland barley brewer's spent grains did not exhibit negative effects on HepG2 cell viability.In addition,as the concentration of the protease hydrolysate of soluble highland barley brewer's spent grains increased,ROS in cells damaged by H2O2 gradually decreased.In fact,5.0 mg/mL(m/V)of the protease hydrolysate reduced ROS in damaged cells by approximately 50%.In addition to serving as a reference,this study provides a theoretical basis for preparing the protease hydrolysate of soluble highland barley brewer's spent grains and illustrates its high-value utilization in healthy food and cosmetics.关键词
青稞酒糟/蛋白酶解液/抗氧化活性/细胞活性Key words
highland barley brewer's spent grains/protease hydrolysate/antioxidant activity/cell viability引用本文复制引用
陈丽花,陈少华,荣玉芝,冯声宝,张叶挺,钟研威,江津津..具有抗氧化活性青稞酒糟蛋白酶解液对H2O2诱导HepG2细胞的保护作用[J].现代食品科技,2024,40(3):28-38,11.基金项目
上海市崇明区科委农业科技攻关项目(CK2022-52) （CK2022-52）
广州市名师工作室(2022MSGZS015) （2022MSGZS015）
广州市大学生创新创业训练计划项目(2022CXCYJH013) （2022CXCYJH013）
