现代食品科技2024，Vol.40Issue(3)：48-55,8.DOI:10.13982/j.mfst.1673-9078.2024.3.0400
原儿茶醛对链脲佐菌素诱导小鼠糖尿病心肌病的改善作用
Effects of Protocatechualdehyde on Streptozotocin Induced Diabetic Cardiomyopathy in Mice
摘要
Abstract
The protective effects of protocatechualdehyde(PCA)on hearts of mice with diabetic cardiomyopathy(DCM)and its possible molecular mechanism were investigated.After the successful construction of DCM mouse models,PCA intervention was made.The heart-to-body mass ratio of mice and the cardiac function were determined.The expression levels of pro-inflammatory factors,troponin I,lactate dehydrogenase(LDH),and creatine kinase(CK)in the myocardial tissues were also determined.Hematoxylin and eosin(HE)staining and Masson staining methods were used to observe the morphological changes of the myocardial tissues.The expressions of nod-like receptor protein 3(NLRP3)in the myocardial tissue and myocardial cells were detected,and the effects of PCA on the survival rate of myocardial cells were evaluated.The results showed that the heart-to-body mass ratio,ejection fraction,and fractional shortening increase by 5.42 mg/g,54.91%,and 28.07%,respectively,after PCA intervention.At the same time,serum LDH,CK,and troponin I concentrations decrease to 538.51 U/L,885.93 U/L,and 221.87 pg/mL,respectively.The levels of tumor necrosis factor-α,interleukin1β,and interleukin-6 also decreased(P<0.05).PCA also inhibited myocardial cytotoxicity and activation of NLRP3 inflammasome induced by high glucose concentrations.PCA can protect the myocardium of DCM mice.Inhibition of the activation of NLRP3 inflammasome may be the way to cardiac improvement.关键词
原儿茶醛/链脲佐菌素/糖尿病心肌病/NLRP3Key words
protocatechualdehyde/streptozotocin/diabetic cardiomyopathy/NLRP3引用本文复制引用
丁萍,叶志明,刘冰,张陆勇..原儿茶醛对链脲佐菌素诱导小鼠糖尿病心肌病的改善作用[J].现代食品科技,2024,40(3):48-55,8.基金项目
广东省普通高校药物早期毒性评价创新团队项目(2018KCXTD016) （2018KCXTD016）
