现代食品科技2024，Vol.40Issue(3)：83-90,8.DOI:10.13982/j.mfst.1673-9078.2024.3.0273
健康婴儿粪便中粪肠球菌的分离鉴定与体外安全性评价
Isolation,Identification,and in Vitro Safety Evaluation of Enterococcus faecalis in the Stool of Healthy Infants
摘要
Abstract
To screen for safe strains of Enterococcus faecalis,stool samples were collected from healthy 1-month-old infants and strains were cultured in MRS medium.A total of 64 E.faecalis strains were identified via 16S rRNA sequencing.Among them,16 were selected for in vitro safety evaluation,and their hemolytic property,antibiotic sensitivity,and six virulence genes were determined.Based on the hemolysis results,the 16 strains of E.faecalis did not exhibit β-hemolysis.Further,the antibiotic sensitivity tests revealed that the strains were highly resistant to tetracycline(56.25%)and erythromycin(43.75%),followed by penicillin(37.50%)and ciprofloxacin(31.25%).No vancomycin-and teicoplanin-resistant strains were found.Eight relatively safe strains were identified based on the above experiments,and five virulence genes:ace,asal,efaA,cylA,and gelE,were subsequently determined,with ace+ efaA+ gelE+ as the dominant mode.Finally,four candidate strains of E.faecalis,namely LX29,LX31,LX41,and LX50,were acquired after comprehensive evaluation.Overall,our findings lay the foundation for subsequent functional research and product development.关键词
粪肠球菌/抗生素耐药性/毒力基因Key words
Enterococcus faecalis/antibiotic resistance/virulence gene引用本文复制引用
桑微,李国花,赵馨,马雨哲,钱卫生,鲁曦..健康婴儿粪便中粪肠球菌的分离鉴定与体外安全性评价[J].现代食品科技,2024,40(3):83-90,8.基金项目
国家自然科学基金项目(31970115) （31970115）
陕西省重点研发计划(2022NY-030) （2022NY-030）
