现代食品科技2024，Vol.40Issue(3)：326-332,7.DOI:10.13982/j.mfst.1673-9078.2024.3.0309
近红外光谱法快速测定晒青毛茶三种儿茶素组分含量
Rapid Determination of Three Catechin Components in Crude Pu-erh Tea by Near Infrared Spectroscopy
摘要
Abstract
In order to achieve rapid detection of the catechin content in crude Pu-erh tea,high performance liquid chromatography(HPLC)was used to determine the contents of three catechin monomers,namely,epigallocatechin gallate(EGCG),epigallocatechin(EGC),and gallic acid(GA),in 50 crude Pu-erh tea samples,and the partial least squares models(PLS)were also established to predict the contents of these catechins by combining the near infrared spectra(NIRS)of the samples.The established models were then verified.The experimental results showed that the coefficient of determination(R2)values of the models for EGCG,EGC,and GA models were 99.99%,99.99%,and 99.92%,respectively.The values of root mean square error of calibration(RMSEC)for EGCG,EGC,and GA were 0.17,0.15,and 0.10,respectively,and the corresponding relative standard deviation(RSD)values were 0.19%,0.27%,and 0.56%,respectively.The results of external validation indicated that the mean absolute errors between the predicted values and actual values of the three models for catechin monomer contents were 0.13,0.12,and 0.07,respectively,and the mean relative errors were 0.17,0.25,and 0.45,respectively.The experimental results show that the near infrared analysis model established in this study has high prediction accuracy and stability,and has potential application value in rapid detection of catechins in crude Pu-erh tea.关键词
晒青毛茶/近红外光谱/儿茶素Key words
Pu-erh crude tea/near infrared spectroscopy(NIRS)/catechin引用本文复制引用
李鑫,沈晓君,王媛媛,刘蕊,闫刚..近红外光谱法快速测定晒青毛茶三种儿茶素组分含量[J].现代食品科技,2024,40(3):326-332,7.基金项目
海南省重点研发计划项目(ZDYF2019178) （ZDYF2019178）
