现代食品科技2024，Vol.40Issue(3)：342-349,8.DOI:10.13982/j.mfst.1673-9078.2024.3.0332
贵州蓝莓蜜TaqMan探针实时荧光PCR检测方法的建立与应用
Establishment and Application of TaqMan Probe Real-time Fluorescent PCR Detection Method for Blueberry Honey in Guizhou
摘要
Abstract
In this study,a highly efficient and accurate method was developed for identifying Guizhou characteristic blueberry honey(from honey bee Apis cerana)based on specific TaqMan probe of real-time fluorescent PCR.Blueberry honey samples(including Apis mellifera)were collected from the blueberry growing areas of Bai zhulin,Maka and Wukaping in Guizhou,meanwhilw,commercial local honey and Canadian blueberry honey were also purchased.Twenty-six blueberry samples at the same flowering stage were collected from the blueberry gardens and the areas around the bee farms in the Baizhulin region of Majiang Town,Guizhou Province.The trnL gene in the sampling plant genome were sequenced.A trnL gene-specific primer of blueberry was designed based on the multiple sequence alignments of trnL gene sequences in the plant genome.TaqMan probe was used for validation.The results showed that the TaqMan probe had high specificity,and the minimum concentration of DNA as low as 0.3 ng/μL can be detected was in blueberry sample using the established TaqMan probe real-time fluorescent PCR detection method.Such an established method was used to test the samples of 11 kinds of commercial honey and Guizhou blueberry honey.It was found that the Ct value of Guizhou blueberry honey was 24～26,the number of blueberry pollen was 800～1 700,and the Ct values of other honey samples were over 30,indicating that this method can effectively distinguish Guizhou blueberry honey from other honeys,thereby having a good application prospect.关键词
蓝莓蜜/实时荧光PCR/TaqMan探针/蜜源植物Key words
blueberry honey/real-time pcr/taqman probe/nectar plant引用本文复制引用
张致豪,孙丽萍,师丰丰,黄家兴,韦小平..贵州蓝莓蜜TaqMan探针实时荧光PCR检测方法的建立与应用[J].现代食品科技,2024,40(3):342-349,8.基金项目
财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-44-SYZ4) （CARS-44-SYZ4）
贵州喀斯特山区优势特色产业提质增效技术集成与示范(2021YFD1100300) （2021YFD1100300）
黔科合支撑[(2021)160] （2021）
