基础医学与临床2024，Vol.44Issue(4)：474-482,9.DOI:10.16352/j.issn.1001-6325.2024.04.0474
壳寡糖上调SRBI核心岩藻糖基化修饰水平减弱小鼠动脉粥样硬化斑块形成
Chitosan oligosaccharides alleviates the formation of atherosclerotic plaque through up-regulating core-fucosylation of SRBI in mice
摘要
Abstract
Objective To explore the mechanism of chitosan oligosaccharides(COS)in reducing atherosclerotic plaque formation from the perspective of protein glycosylation modification.Methods Totally 40 ApoE-/-mice were randomly divided into control group and COS group.The control group was given a high-fat diet for 12 weeks,and COS group was given a high-fat diet plus COS(gavage per day,500 mg/kg)for 12 weeks.Serum lipid detection,HE staining and Oil red O staining were used to detect plaque formation.Lectin chip,liquid chromatography tan-dem-mass spectrometry and ELISA were used to detect potential changes of glycoprotein in serum.Cholesterol ester outflow and free cholesterol ester determination experiment were used to evaluate the effect of changes in scavenger receptor class B type Ⅰ(SRBI)protein glycosylation modification site on cholesterol effluence in macrophages.Results COS significantly reduced the level of TC and LDL-C(P＜0.05)in mice,but had no effect on the level of TG,HDL-C,ApoA1 and ApoB100.The intima thickness and plaque size of the aorta were significantly thinner and smaller(P＜0.05)in the COS group compared with the control group.The molecular weight of lens culinaris ag-glutinin(LCA)binding protein with the most obvious change is 80-90 ku,and SRBI was one of them.COS promo-ted the cholesterol outflow and inhibited the accumulation of free cholesterol ester in RAW264.7 cell(P＜0.05).Knockdown or glycosylation site mutation with SRBI inhibited cholesterol outflow caused by COS,and increased the accumulation of intracellular free cholesterol(P＜0.05).Conclusions COS promotes lipid efflux by increasing SRBI glycosylation and expression,thereby alleviating atherosclerotic plaque formation.关键词
壳寡糖/清道夫受体B类成员1/核心岩藻糖基化/动脉粥样硬化Key words
chitosan oligosaccharides/scavenger receptor class B member Ⅰ(SRBI)protein/core-fucosylation/atherosclerosis分类
医药卫生引用本文复制引用
陈林木,黄云秀..壳寡糖上调SRBI核心岩藻糖基化修饰水平减弱小鼠动脉粥样硬化斑块形成[J].基础医学与临床,2024,44(4):474-482,9.基金项目
广东省医学科研基金(B2020116) （B2020116）
中山市第三批社会公益与基础研究专项(2020B3004) （2020B3004）
中山市人民医院一类青年骨干科研启动经费(SGKY2022006) （SGKY2022006）
