DHA对人脑胶质瘤T98G细胞迁移和侵袭的影响机制OACSTPCD
The mechanism of DHA's effect on the migration and invasion of human glioma T98G cells
目的 探究双氢青蒿素(DHA)对人脑胶质瘤T98G细胞迁移和侵袭的影响机制.方法 体外培养人脑胶质瘤T98G细胞,将其分为对照组、低DHA组(10 mg/L DHA)、中DHA组(20 mg/L DHA)、高DHA组(40 mg/L DHA)、阳性药物组(50 mg/L 5-氟尿嘧啶)和抑制剂组(20 mg/L DHA+20 μmol/L LY294002).采用CCK-8、细胞黏附实验、Transwell小室及蛋白免疫印迹法分别对细胞增殖活力、黏附、迁移、侵袭能力及上皮间质转化(EMT)、磷脂酰肌醇3-激酶(PI3K)/丝苏氨酸蛋白激酶(Akt)通路相关蛋白的表达水平进行分析.结果 随着细胞处理时间的递增,各组T98G细胞增殖活力呈递增趋势(P<0.05).与对照组比较,其他各组T98G细胞增殖活力、黏附、迁移和侵袭能力、p-PI3K、p-Akt、p-ERK、N-cadherin、Vimentin和FN蛋白表达均显著降低(P<0.05),而E-cadherin蛋白表达显著升高(P<0.05),且低、中、高DHA组呈剂量依赖性降低或升高(P<0.05).与低、中DHA组比较,阳性药物组、抑制剂组细胞增殖活力、黏附、迁移和侵袭能力、p-PI3K、p-Akt、p-ERK、N-cad-herin、Vimentin和FN蛋白显著降低(P<0.05),而 E-cadherin蛋白表达显著升高(P<0.05).结论 DHA抑制人脑胶质瘤T98G细胞的黏附、迁移、侵袭和EMT,其机制可能是通过抑制PI3K/Akt通路的信号转导发挥作用.
Aim To investigate the mechanism of the effects of dihydroartemisinin(DHA)on migration and invasion of hu-man glioma T98G cells.Methods Human glioma T98G cells were cultured in vitro,and were divided into control group,low DHA group(10 mg/L DHA),medium DHA group(20 mg/L DHA),high DHA group(40 mg/L DHA),positive drug group(50 mg/L 5-fluorouracil)and inhibitor group(20 mg/L DHA+20 μmol/L LY294002).CCK-8,cell adhesion assay,Transwell chamber and Western blotting were used to analyze cell proliferation,adhesion,migration,invasion,EMT and phosphatidylinositol 3-kinase(PI3K)/seronine protein kinase(Akt)path-related protein expression levels in each group,respectively.Results With the cell processing time increased,the proliferation activity of T98G cells in each group showed a trend of increase(P<0.05).Compared with the control group,the proliferation activity,adhesion,migration and invasion ability,and the expression of p-PI3K,p-Akt,p-ERK,N-cadherin,Vimentin,and FN protein of T98G cells in other groups were significantly decreased(P<0.05),while the expres-sion of E-cadherin protein is significantly increased(P<0.05),and the low,medium,and high DHA groups showed a dose-dependent decrease or increase(P<0.05).Compared with the low and medium DHA groups,the proliferation activity,adhesion,migration and invasion ability,and the expression of p-PI3K,p-Akt,p-ERK,N-cadherin,Vimentin,and FN protein of T98G cells in the posi-tive drug group and inhibitor group were significantly decreased(P<0.05),while the expression of E-cadherin protein was significantly increased(P<0.05).Conclusion DHA inhibits adhesion,migration,invasion and EMT of human glioma T98G cells,possibly by inhibiting the signal transduction of PI3K/Akt pathway.
王克;范志刚;包志军
三二○一医院 神经外科,陕西汉中 723000三二○一医院 肿瘤科,陕西汉中 723000
临床医学
脑胶质瘤双氢青蒿素PI3K/Akt黏附迁移侵袭T98G细胞
glioma cancerDHAPI3K/AktadhesionmigrationinvasionT98G cells
《中南医学科学杂志》 2024 (002)
182-186 / 5
陕西省科学技术厅2021年度科技计划项目(2021SF-044)
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