中南医学科学杂志2024，Vol.52Issue(2)：182-186,5.DOI:10.15972/j.cnki.43-1509/r.2024.02.006
DHA对人脑胶质瘤T98G细胞迁移和侵袭的影响机制
The mechanism of DHA's effect on the migration and invasion of human glioma T98G cells
摘要
Abstract
Aim To investigate the mechanism of the effects of dihydroartemisinin(DHA)on migration and invasion of hu-man glioma T98G cells.Methods Human glioma T98G cells were cultured in vitro,and were divided into control group,low DHA group(10 mg/L DHA),medium DHA group(20 mg/L DHA),high DHA group(40 mg/L DHA),positive drug group(50 mg/L 5-fluorouracil)and inhibitor group(20 mg/L DHA+20 μmol/L LY294002).CCK-8,cell adhesion assay,Transwell chamber and Western blotting were used to analyze cell proliferation,adhesion,migration,invasion,EMT and phosphatidylinositol 3-kinase(PI3K)/seronine protein kinase(Akt)path-related protein expression levels in each group,respectively.Results With the cell processing time increased,the proliferation activity of T98G cells in each group showed a trend of increase(P<0.05).Compared with the control group,the proliferation activity,adhesion,migration and invasion ability,and the expression of p-PI3K,p-Akt,p-ERK,N-cadherin,Vimentin,and FN protein of T98G cells in other groups were significantly decreased(P<0.05),while the expres-sion of E-cadherin protein is significantly increased(P<0.05),and the low,medium,and high DHA groups showed a dose-dependent decrease or increase(P<0.05).Compared with the low and medium DHA groups,the proliferation activity,adhesion,migration and invasion ability,and the expression of p-PI3K,p-Akt,p-ERK,N-cadherin,Vimentin,and FN protein of T98G cells in the posi-tive drug group and inhibitor group were significantly decreased(P<0.05),while the expression of E-cadherin protein was significantly increased(P<0.05).Conclusion DHA inhibits adhesion,migration,invasion and EMT of human glioma T98G cells,possibly by inhibiting the signal transduction of PI3K/Akt pathway.关键词
脑胶质瘤/双氢青蒿素/PI3K/Akt/黏附/迁移/侵袭/T98G细胞Key words
glioma cancer/DHA/PI3K/Akt/adhesion/migration/invasion/T98G cells分类
医药卫生引用本文复制引用
王克,范志刚,包志军..DHA对人脑胶质瘤T98G细胞迁移和侵袭的影响机制[J].中南医学科学杂志,2024,52(2):182-186,5.基金项目
陕西省科学技术厅2021年度科技计划项目(2021SF-044) （2021SF-044）
