中南医学科学杂志2024，Vol.52Issue(2)：193-197,5.DOI:10.15972/j.cnki.43-1509/r.2024.02.008
白花蛇舌草诱导活性氧类物质抑制慢性髓系白血病K562细胞增殖
Mechanism of hedyotis diffusa willd inhibiting the proliferation of K562 cells in chronic mye-loid leukemia by inducing reactive oxygen species
摘要
Abstract
Aim To explore the effects of hedyotis diffusa willd(HDW)on the proliferation of K562 cells in chronic mye-loid leukemia(CML)by inducing reactive oxygen species(ROS).Methods ROS content in K562 cells treated with HDW was detected by flow cytometry.K562 cells were divided into control group,HDW group,N-acetyl-L-cysteine group(NAC group)and HDW+NAC group.ROS content,cells activity,morphology,proliferation and distribution of cells cycle in different groups were measured.The expressions of cells cycle-related proteins,phosphorylated phosphatidylinositol 3-kinase(p-PI3K)and serine threo-nine kinase(p-Akt)proteins were detected by Western blotting.Results HDW could increase ROS content in K562 cells(P<0.05).In control group and NAC group,there were a large number of trophochrome cells,and there was no significant change in cells morphology.In HDW group and HDW+NAC group,trophochrome cells were reduced,staining cells were increased,and there was chromatin aggregation and nuclear membrane disintegration.Compared with control group,ROS content,proportion of cells in G1 phase and expressions of p21 and p53 proteins were increased,while proportion of cells in S phase and expressions of cyclin D1,cyclin-dependent kinase 4(CDK4),p-PI3K and P-Akt proteins were decreased in HDW group(P<0.05).NAC intervention could partially reverse the effect of HDW(P<0.05).Conclusion HDW can decrease proliferation ability of K562 cells by inducing the increase of ROS,which is related to cell cycle arrest and regulating activity of PI3K/Akt pathways.关键词
慢性髓系白血病/K562细胞/白花蛇舌草/细胞增殖/活性氧Key words
chronic myeloid leukemia/K562 cell/hedyotis diffusa willd/cell proliferation/reactive oxygen specie分类
医药卫生引用本文复制引用
唐丽,于慧静,王德文..白花蛇舌草诱导活性氧类物质抑制慢性髓系白血病K562细胞增殖[J].中南医学科学杂志,2024,52(2):193-197,5.基金项目
威海市文登区科技发展计划项目(20210453) （20210453）
