食品工业科技2024，Vol.45Issue(8)：39-46,8.DOI:10.13386/j.issn1002-0306.2023080137
铁皮石斛中性多糖的分离纯化及抗炎作用
Isolation and Purification of Polysaccharide and Anti-inflammatory Effect of Dendrobium officinale Neutral Polysaccharide
林晓亮 1谢玲娜 2陈梦香 2梁明 1梁逸恒 2杜志云2
作者信息
- 1. 无限极(中国)有限公司,广东广州 510623
- 2. 广东工业大学生物医药学院,广东广州 510006
- 折叠
摘要
Abstract
Objective:To isolate and purify polysaccharides from Dendrobium officinale,characterize their structure and explore their anti-inflammatory effects.Methods:Polysaccharides from Dendrobium officinale were obtained by water extraction and alcohol precipitation.DOP-1 was purified by DEAE-52 cellulose ion exchange resin and Sephadex G-100.Ultraviolet scanning,GC,high-performance gel chromatography,infrared spectroscopy and nuclear magnetic resonance scanning were used to analyze the structure of DOP-1 polysaccharide,and the inhibitory effect of DOP-1 on the proliferation of RAW 264.7 cells was determined.And its effect on the expression of NO,TNF-α and IL-1β in RAW264.7 cells induced by LPS.Results:DOP-1 was a homogeneous polysaccharide composed of rhamnose,mannose,glucose and galactose with a monosaccharide molar ratio of 0.6∶9.0∶2.7∶2.9 and a molecular weight of 59.2 kDa.DOP-1 did not significantly inhibit the proliferation of RAW 264.7 cells.Compared with the model group,DOP-1 significantly inhibited the expression of NO,TNF-α and IL-1β.Conclusions:The polysaccharides of Dendrobium officinaleobtained in this study had clear structure and good anti-inflammatory activity,and the extraction conditions were feasible.This study would provide theoretical basis for the development and utilization of polysaccharides of Dendrobium officinale with anti-inflammatory effect.关键词
铁皮石斛/中性多糖/分离纯化/结构分析/抗炎作用Key words
Dendrobium officinale/neutral polysaccharide/isolation and purification/structural analysis/anti-inflammatory effect分类
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林晓亮,谢玲娜,陈梦香,梁明,梁逸恒,杜志云..铁皮石斛中性多糖的分离纯化及抗炎作用[J].食品工业科技,2024,45(8):39-46,8.
