水生生物学报2024，Vol.48Issue(5)：725-733,9.DOI:10.7541/2024.2023.0341
盐度胁迫对大黄鱼能量代谢与线粒体自噬的影响
SALINITY STRESS ON ENERGY METABOLISM AND MITOCHONDRIAL AUTOPHAGY IN LARGE YELLOW CROAKER
摘要
Abstract
Large yellow croaker(Larimichthys crocea)is an important marine economic fish in China,however,it faces challenges such as insufficient aquaculture water,severe water pollution,and frequent occurrence of diseases.Expand-ing cultivation from the inner bay to the outer sea can address these issues,but varying water salinity across sea areas poses a hurdle.Thus,it is necessary to understand the impact of salinity changes on fish physiology.This study aimed to compare how high and low salinity affect energy metabolism and mitophagy in the liver of large yellow croaker and elucidate the underlying mechanism.Fish weighing(53.46±1.47)g were obtained from Fufa Aquatic Co.Ltd.(Ningde,China)and randomly placed in 400 L fiberglass tanks(9 tanks)with seawater salinity at 12,25 or 40.This setup was maintained for 1d,3d and 7d,with three replicates for each salinity group.Measurements included oxidative damage markers(radical oxygen species(ROS)),energy metabolism indicators(adenosine triphosphate(ATP)and lactate contents;pyruvate kinase(PK),lactic dehydrogenase(LDH),succinate dehydrogenase(SDH),malate dehydrogenase(MDH),ATP synthase(F-ATP),carnitine palmitoyl transterase-1(CPT-1),acetyl-CoA carboxylase(ACC)and adeno-sine 5'-monophosphate(AMP)-activated protein kinase(AMPK)enzyme activities),and mitochondrial autophagy indi-cators(microtubule-associated protein light chain 3(LC3α),PTEN induced putative kinase 1(PINK1),Parkin,mito-fusin 2(Mfn2)and forkhead box class O3(FoxO3)gene expression levels).The results showed that tricarboxylate cyclase enzyme(SDH,MDH and F-ATP)activities and mitophagy gene(LC3α,PINK1,Parkin,Mfn2)expression levels in the high-salt group were significantly higher than those in the low-salt group on day 1,indicating that increased energy consumption and enhanced mitophagy to cope with early high-salt stress.The high-salt group increased fatty acid β oxidase enzyme(CPT-1)activities,and reduced F-ATP activity and LC3 α gene expression levels on day 3,resulting in increased lactate and ROS contents.F-ATP activity and LC3α gene expression levels in the high-salt group were lower while ROS and lactate contents and acetyl ACC activity were higher than those in the low-salt group on day 7,suggesting a shift toward decreased aerobic metabolism,reduced mitophagy,and increased anaerobic metabolism,leading to insufficient energy supply,loss of mitophagy function,and exacerbated oxidative damage.Furthermore,gene expressions of AMPK and FoxO3 positively correlated with mRNA levels of energy metabolism and mitophagy related genes,respectively,indicating that AMPK and FoxO3 played important roles in regulating energy metabolism enzyme activities and mitophagy gene expressions during the salinity stress.In conclusion,research results highlight the differences in the effects of high and low salinity on energy metabolism and mitophagy in large yellow croaker.These findings provide basic data for formulating salinity regulation plans and selecting suitable aquaculture water bodies for large yellow croaker.关键词
能量代谢/线粒体自噬/盐度胁迫/大黄鱼Key words
Energy metabolism/Mitophagy/Salinity stress/Larimichthys crocea分类
生物科学引用本文复制引用
曾霖,熊逸飞,宋炜,谢正丽,王永红..盐度胁迫对大黄鱼能量代谢与线粒体自噬的影响[J].水生生物学报,2024,48(5):725-733,9.基金项目
青岛海洋科技中心山东省专项经费(2022QNLM30001) （2022QNLM30001）
国家重点研发计划(2022YFD2401102) （2022YFD2401102）
中国水产科学研究院基本科研业务费(2020TD76)资助[Supported by the Marine S & T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(2022QNLM30001) （2020TD76）
National Key Research and Development Program of China(2022YFD 2401102) （2022YFD 2401102）
Central Public-interest Scientific Institution Basal Research Fund,CAFS(2020TD76)] （2020TD76）
