新疆农业科学2024，Vol.61Issue(3)：607-614,8.DOI:10.6048/j.issn.1001-4330.2024.03.010
向日葵副产物中优势乳酸菌和纤维素分解菌的生理生化特征分析
Physiological and biochemical characteristics of dominant lactic acid bacteria and cellulolytic bacteria in sunflower By-Products
摘要
Abstract
[Objective]This study aims to provide a basis for fermented feeds by sunflower by-products.[Methods]The dominant lactic acid bacteria and cellulolytic bacteria attached to the surface of sunflower by-products were isolated,extracted and identified,and the physiological and biochemical characteristics of the dominant bacteria were analyzed.[Results]3 strains of lactic acid bacteria and 4 strains of cellulolytic bacte-ria were isolated.The 3 strains of lactic acid bacteria obtained were Enterococcus mundtii.Among the 4 strains of cellulolytic bacteria,strains Z2 and Z13 were Bacillus velezensis,X14 was Bacillus aryabhattai,and X4 was Ba-cillus amyloliquefaciens.3 strains of lactic acid bacteria grew well under the conditions of 4,10,30 and 45℃,3%and6.5%NaCl,grew at pH3.5-9,and did not grow at pH3;among the4 strains of cellulolytic bacteri-a,the ratio of transparent circle diameter(D)/colony diameter(d)and enzyme activity in order of size were Bacillus amyloliquefaciens>Bacillus velezensis>Bacillus aryabhattai.[Conclusion]Enterococcus mundtii has strong salt tolerance and a wide range of temperature adaptation,but its acid-producing ability is signifi-cantly weaker than that of Lactobacillus plantarum.In the actual weight loss rate of the product,the effect of in-oculation with Bacillus amyloliquefaciens is the best.关键词
向日葵副产物/乳酸菌/纤维素分解菌/分离/鉴定/酶活性Key words
sunflower byproduct/lactic acid bacteria/cellulolytic bacteria/isolation/identification/en-zymatic activity分类
农业科技引用本文复制引用
李肖,陈永成,黄嵘峥,许平珠,张凡凡,马春晖..向日葵副产物中优势乳酸菌和纤维素分解菌的生理生化特征分析[J].新疆农业科学,2024,61(3):607-614,8.基金项目
农业农村部:国家现代农业产业技术体系项目(CARS) （CARS）
新疆维吾尔自治区优质饲草产业技术体系项目(2022XJSC-Z-02) Ministry of Rural Agriculture:China Agriculture Research System(CARS) （2022XJSC-Z-02）
Xinjiang Uygur Autonomous Region High-quality forage industry technology system project(2022XJSC-Z-02) （2022XJSC-Z-02）
