中国食品学报2024，Vol.24Issue(3)：122-129,8.DOI:10.16429/j.1009-7848.2024.03.012
山梨酸钾辅助HPTS对芽孢的杀菌动力学及应用研究
Study on Bactericidal Kinetics and Application of Potassium Sorbate Assisted HPTS to Spores
摘要
Abstract
In this paper,the inactivation kinetics of potassium sorbate-assisted high pressure thermal sterilization(HPTS)on Bacillus subtilis spores were studied.First-order kinetics model,Weibull and Log-logistic kinetics models were used to fit the inactivation curve of B.subtilis spores by potassium sorbate-assisted HPTS.By analysis of precision factor(Af),bias factor(Bf),root mean square error(RMSE),coefficient of determination(R2),In the three models,Log-logistic Kinetic model fitted best the spore sterilization effect of potassium sorbate-assisted HPTS(R2=0.986).When potassium sorbate(0.2 g/100 mL)assisted HPTS(600 MPa,75 ℃,25 min),the inactivation effect was the best,and the spore inactivation was 6.10 lg(CFU/mL).Potassium sorbate assisted HPTS treatment was applied to the inactivation of spores in muskmelon juice,it was found that the browning degree was significantly reduced,after 28 days of storage,the absorbance of potassium sorbate(0.1 g/100 mL)assisted HPTS(600 MPa,75℃,25 min)at 420 mn reached 0.093,The absorbance of the HPTS-treated control sample was 0.058.Scanning electron microscope was used to observe the microstructure of Bacillus subtilis spores in muskmelon juice before and after treatment,and it was found that the mor-phological structure of the spores changed after treatment.In conclusion,potassium sorbate assisted HPTS has significant application value.关键词
山梨酸钾/HPTS/枯草杆菌芽孢/杀菌动力学Key words
potassium sorbate/HPTS/Bacillus subtilis spores/bactericidal kinetics引用本文复制引用
武思睿,杨杰,李佳佳,王旭娟,辛伟山,章中..山梨酸钾辅助HPTS对芽孢的杀菌动力学及应用研究[J].中国食品学报,2024,24(3):122-129,8.基金项目
国家自然科学基金项目(31760474) （31760474）
