中国食品学报2024，Vol.24Issue(3)：258-267,10.DOI:10.16429/j.1009-7848.2024.03.026
原料乳及牧场环境中的微生物多样性及嗜冷菌污染源分析
Microbial Diversity and Contamination Sources of Psychrophilic Bacteria in Raw Milk and in Pastures Environment
摘要
Abstract
Excessive psychrophilic bacteria in raw milk can produce high levels of heat-stable protease and lipase,both of which are important causes of quality problems when cow's milk enters the shelf life.In order to explore the microbial diversity of cows'living environment in pasture and different stages in the raw milk circulation process,the pollution source of psychrophilic bacteria in the pasture was found out.In this study,we collected five samples in a large-scale dairy farm in Hebei Province:TMR feed,cowshed pad soil,the first three milks,the normal milk after the first three milks,and the raw milk in the milk tank,and analyzed the bacterial community structure,psychrophilic species,abun-dance of psychrophilic bacteria in all samples by using metagenomic shotgun sequencing technology.The results showed that the mainly microbial were Proteobacteria,Firmicutes,and Actinobacteria at phylum level.At the genus level,the main genera were Anaplasma,Streptococcus,Enterococci,Spirospirita,Bacillus,and Pseudomonas.The soil of the barn contains 99.63％and 96.39％of the species of Pseudomonas in the normal milk and the total milk of the pasture storage tank,indicating that the soil of the barn mat is a potential source of pollution of various types of Pseudomonas.关键词
宏基因组测序技术/微生物多样性/嗜冷菌/假单胞菌/污染来源Key words
metagenomic shotgun sequencing technology/microbial diversity/psychrophilic bacteria/Pseudomonas/sources of pollution引用本文复制引用
胡少震,逯刚,喻东威,吕加平,张书文,李莲瑞,逄晓阳..原料乳及牧场环境中的微生物多样性及嗜冷菌污染源分析[J].中国食品学报,2024,24(3):258-267,10.基金项目
国家自然科学基金项目(32172190,31871833) （32172190,31871833）
呼和浩特市科技计划项目(2021-农-重-1) （2021-农-重-1）
