中国食品学报2024，Vol.24Issue(3)：268-275,8.DOI:10.16429/j.1009-7848.2024.03.027
大鲵骨酸溶性和胃蛋白酶溶性胶原蛋白的提取与表征
Extraction and Characterization of Acid-Soluble and Pepsin-Soluble Collagen from Giant Salamander Bone
摘要
Abstract
The giant salamander,as a precious amphibian,is rich in nutritional value.To fully and efficiently utilize its processing by-products,acid-soluble collagen(ASC)and pepsin-soluble collagen(PSC)from giant salamander bone were isolated and characterized.The yields of salamander bone ASC and PSC were 10.71％and 13.11％,respectively.The stable triple helical structure of collagen was revealed by Fourier transform infrared spectroscopy.SDS-PAGE electro-pherograms showed that both samples were type I collagen with high levels of imino acids(179-187/1 000 residues).Their solubility was highest at pH 6.The thermal denaturation temperature(45.78-46.51 ℃)was significantly higher than that of marine and freshwater fish species.Under SEM,the collagen showed dense flakes and irregular holes.These re-sults show that the characteristics of giant salamander bone collagen are similar to mammalian collagen,and it has the potential to become its substitute,which provides a basis for its subsequent research and development and utilization.关键词
大鲵骨/胶原蛋白/提取/表征Key words
giant salamander bone/collagen/extraction/characterization引用本文复制引用
宫萱,包建强,黄可承,陈彦婕,唐嘉诚..大鲵骨酸溶性和胃蛋白酶溶性胶原蛋白的提取与表征[J].中国食品学报,2024,24(3):268-275,8.基金项目
水产动物遗传育种中心上海市协同创新中心项目(ZF1206) （ZF1206）
上海市科委工程中心建设项目(11DZ2280300) （11DZ2280300）
