中国比较医学杂志2024，Vol.34Issue(2)：9-15,7.DOI:10.3969/j.issn.1671-7856.2024.02.002
骨桥蛋白通过LGALS3BP调控PI3K/AKT通路促进肝癌细胞迁移
Osteopontin activates the PI3K/AKT pathway by upregulating LGALS3BP in promotion of hepatoma cell migration
摘要
Abstract
Objective To investigate the effect and mechanism of osteopontin(OPN)in hepatoma cell migration through galectin-3 binding protein(LGALS3BP).Methods Human hepatoma cell lines SMMC-7721,SMMC-P(stably transfected with empty eukaryotic expression vectors),and SMMC-OPN(stably transfected with the OPN gene)were cultured.mRNA expression levels of OPN and LGALS3BP were measured by RT-qPCR.Western blot assays were used to analyze the relative protein expression of OPN and LGALS3BP and PI3K/AKT pathway.Wound healing assays were performed to explore the cell migration ability.After transfection with LGALS3BP-targeting small interfering RNA(si-LGALS3BP)or negative control small RNA(si-NC)into SMMC-OPN cells,cell migration and relative expression of PI3K/AKT pathway-related proteins were assessed.Results Compared with SMMC-7721 and SMMC-P,the migratory ability of SMMC-OPN cells was significantly reinforced,and expression of LGALS3BP was obviously upregulated at both mRNA and protein levels.Moreover,relative expression of p-PI3K/PI3K and p-AKT/AKT proteins was significantly increased.Wound healing assays showed that the si-LGALS3BP obviously suppressed the migratory ability of SMMC-OPN cells.Furthermore,relative expression of p-PI3K/PI3K and p-AKT/AKT proteins in SMMC-OPN cells was significantly decreased after transfection of si-LGALS3BP.Conclusions OPN activates the PI3K/AKT pathway by upregulating LGALS3BP expression to promote hepatoma cell migration.关键词
骨桥蛋白/LGALS3BP/PI3K/AKT通路/肝癌/细胞迁移Key words
osteopontin/LGALS3BP/PI3K/AKT pathway/liver cancer/cell migration分类
医药卫生引用本文复制引用
邓林林,安日问,赵方新,林婷,刘翠华,红梅,武建强,张烜..骨桥蛋白通过LGALS3BP调控PI3K/AKT通路促进肝癌细胞迁移[J].中国比较医学杂志,2024,34(2):9-15,7.基金项目
国家自然科学基金(81660482) （81660482）
内蒙古自治区自然科学基金(2022MS08009) （2022MS08009）
内蒙古自治区卫生健康科技计划项目(202202128). （202202128）
