中国比较医学杂志2024，Vol.34Issue(2)：55-62,8.DOI:10.3969/j.issn.1671-7856.2024.02.007
维生素D对自发性糖尿病大鼠肠道菌群结构和多样性的影响
Effect of vitamin D on the structure and diversity of intestinal flora in spontaneously diabetic rats
摘要
Abstract
Objective To investigate the effect of vitamin D(VD)on intestinal flora in spontaneously diabetic rats.Methods Zucker diabetic fatty rats(ZDF rats)were randomised to control(Con)group,VD control(VD)group,model(T2DM)group,and VD intervention(VD+T2DM)group.Fasting blood glucose profiles and oral glucose tolerance levels were determined in rats of each group.16S rDNA sequencing was used to assess changes in rat intestinal flora.OTU analysis(Venn diagram),α diversity analysis(chao1,observed species,PD whole tree,and shannon and simpson),βdiversity analysis(principal coordinate analysis(PCoA)),flora structure,and colony species variability analysis(linear discriminant analysis and influence factor(LEfSe)analysis)were also performed.Results VD intervention significantly improved fasting blood glucose levels and insulin resistance in T2DM rats(P＜0.05).α diversity showed no significant differences in chao1,observed species,PD whole tree,and shannon and simpson indices between T2DM and VD+T2DM groups(P＞0.05).β diversity analysis showed that the VD+T2DM group had more species similarity to the Con group than the T2DM group.The dominant bacteria of rat intestinal flora in each group were significantly different.In comparison to the T2DM group,the VD+T2DM group showed a decrease in abundance of Bacteroidetes and increases in abundances of Firmicutes and Clostridium XIVa.Conclusions VD improves fasting glucose elevation and insulin resistance in T2DM rats.VD improves the structure of intestinal flora,decreases Bacteroidetes,and elevates Firmicutes and Clostridium XIVa abundances in T2DM rats.关键词
维生素D/糖尿病/肠道菌群/16S rDNA测序/血糖/胰岛素抵抗Key words
vitamin D/diabetes/intestinal flora/16S rDNA sequencing/blood glucose/insulin resistance分类
医药卫生引用本文复制引用
陈琳,谭洪兴,郝丽萍,李艳艳..维生素D对自发性糖尿病大鼠肠道菌群结构和多样性的影响[J].中国比较医学杂志,2024,34(2):55-62,8.基金项目
深圳医学重点学科建设经费资助(SZXK065) （SZXK065）
国家自然科学基金(81703226). （81703226）
