1800 MHz电磁辐射暴露对大鼠外周血单个核细胞代谢组的影响OACSTPCD

Objective To analyze the effect of electromagnetic radiation exposure on the metabolite change of peripheral blood mononuclear cells(PBMCs)in rats.Methods A total of 24 SD rats were randomly divided into the electromagnetic radiation group(EMR group)and the Sham group.The rats in the EMR group were placed in an electromagnetic reverberation box with a center frequency of 1 800 MHz and an average power density of 284.6 V/m,and were exposed for 10 minutes every day,and continuously for 7 days;the rats in the Sham group were given an irradiation power of 0 W,and other conditions and operations were the same as those of the EMR group.After exposure for 7 days,peripheral blood of rats was collected and PBMCs were isolated for liquid chromatography-tandem mass spectrometry(LC-MS/MS)untargeted metabolomics analysis.Principal component analysis was conducted using partial least squares-discriminant analysis(PLA-DA),univariate analysis was used to screen differential metabolites,and then combined with mzCloud and ChemSpider databases to identify the detected metabolites,and a metabolic pathway was constructed to analyze the effects of electromagnetic radiation exposure on the metabolome of PBMCs in rats.Results A total of 2 017 differential metabolites were detected from the PBMCs under the positive and negative ion modes of the SD rats in the EMR group and the Sham group,among which 1 611 metabolites were obtained under the positive ion mode,with 847 up-regulated metabolites and 764 down-regulated metabolites,and 406 metabolites were obtained under the negative ion mode,with 220 up-regulated metabolites,and 186 down-regulated metabolites.Differential metabolites detected in the positive and negative ion modes included amino acids,peptides,organic acids,bile acids,and so on.KEGG enrichment analysis showed that there were 64 metabolic pathways involved in the differential metabolites under the positive and negative ion modes,including amino acid metabolism,lipid metabolism,cell growth,cell proliferation and cell death,oxidative stress,and inflammatory response.Conclusion Simulated occupational environment exposure to electromagnetic radiation has a significant effect on the metabolome of PBMCs in SD rats,and its differential metabolites are expected to be biomarkers of electromagnetic exposure.