陕产附子炮制前后急性毒性及强心作用研究OACSTPCD

Objective:To study the acute toxicity and cardiotonic effects of Aconiti Lateralis radix praeparata produced in Shaanxi before and after processing.Methods:The acute toxicity of raw Aconiti Lateralis radix praeparata and self-made Heishun tablet was compared through the measurement of LD50 in raw Aconiti Lateralis radix praeparata and the maximum tolerance experiment of self-made Heishun tablet.By replicating a chronic heart failure rat model,the body mass,cardiac index,hemodynamic indicators,serum BNP and cTnI levels,and histopathological changes were measured to compare their cardiotonic effects.Results:the LD50 of raw Aconiti Lateralis radix praeparata was 9.45 g/kg,the maximum tolerable dose of Heishun tablet was 27.39 g/kg.The toxicity of Aconiti Lateralis radix praeparata was reduced after being processed.Compared with the model group,the symptoms and signs of the rats in self-made Heishun tablet group were improved,the heart and left ventricular indexes were reduced;the LVEDP was significantly reduced,the LVSP was increased,and + dp/dtmax and-dp/dtmax were significantly increased(P<0.05,P<0.01).Serum BNP and cTnI levels were significantly reduced(P<0.05),and the cardiac muscle fibers were arranged relatively tightly.The nuclei were clearly visible,but there were some ruptures,a small amount of vacuoles were present,no inflammatory cell infiltration was found,and there were significant differences in the indicators.The indexes of raw Aconiti Lateralis radix praeparata group improved to a certain extent,but there was no statistical significance(P>0.05).Conclusion:After processing,the toxicity of raw Aconiti Lateralis radix praeparata is reduced,and the self-made Heishun tablets have significantly cardiotonic enhancing effect compared to raw Aconiti Lateralis radix praeparata.