桑黄素通过抑制ERK1/2-p38信号通路介导对老年大鼠骨骼保护作用OA北大核心CSTPCD

[Objective]To investigate the effects of morin treatment on bone metabolism and bone mass in aged rats,and to clarify the possible mechanism.[Methods]Ten young female Sprague-Dawley rats(3 months old)and 20 old fe-male Sprague-Dawley rats(24 months old)were randomly divided into three groups:Control group(CON,10 young rats);Model group(MOD,10 young rats);10 old rats and SangHuangSu Group(SSS,10 old rats).During the experi-ment,the SSS group received intraperitoneal injection of morin(10 mg/kg)daily.The treatment lasted for 12 weeks.Af-ter treatment,Micro-CT,HE stained sections,serological tests and Western blot were used to observe the treatment effect and possible mechanism.[Results]After 12 weeks of treatment,compared with MOD group,the number and density of bone trabeculae in SSS group were significantly improved.The BMD,Conn.D,Tb.N,Tb.Th and Tb.Sp of the left femur in the SSS group were significantly better than those in the MOD group(P<0.05).After 12 weeks of treatment,the levels of CTX-1,osteocalcin,TRACP-5b and PINP in SSS group were significantly lower than those in MOD group(P<0.05).Compared with the MOD group,the ERK1/2-p38 signal pathway was significantly inhibited and the levels of ERK1/2 and p38 were significantly decreased in the SSS group(P<0.05).[Conclusion]Morin pigment mediates the protective effect on the bones of aged rats by inhibiting the ERK1/2-p38 signaling pathway and reducing bone turnover.