掌叶覆盆子HMGR基因家族的鉴定及表达分析OA北大核心CSTPCD

3-hydroxy-3-methylglutaryl coenzyme A reductase(HMGR)is one of the most significant enzymes for the terpene biosynthesis in medicinal plants.To explicit the function of HMGR gene family in Rubus chingii Hu,the classification,gene structure,chromosomal distribution,and promoter information of RcHMGR members were systematically analyzed through bioinformatical methods.The expression levels were comparatively detected in different tissues,different stages of fruit development,and under the treatment of methyl jasmonate(MeJA).The results demonstrated that a total of 7 RcHMGR members were mined,which were located on 7 chromosomes,encoding 444-601 aa protein sequences.RcHMGR were divided into four subfamilies,located in the endoplasmic reticulum,and harbored the highly conserved HMGR domains.Multiple cis-acting elements including hormone,light,and low-temperature responses were presented in the promoter regions of RcHMGR family genes.RcHMGR family genes were differentially expressed in different tissues(roots,stems,leaves,flowers,and fruits),different fruit development stages(small green fruits,big green fruits,yellow fruits,and red fruits),and treatment of MeJA.Among them,RcHMGR5 was highly expressed in fruits of R.chingii,and induced by MeJA,which was coincided with the accumulation of carotenoids,suggesting that RcHMGR5 might be a critical enzyme involved in carotenoid biosynthesis.These results laid a foundation for in-depth study on the role of RcHMGR family genes in R.chingii.