基础医学与临床2024，Vol.44Issue(5)：613-618,6.DOI:10.16352/j.issn.1001-6325.2024.05.0613
利用不同方法将小分子NADPH转染进入细胞的比较
Application of different methods used to transfect small molecule NADPH into cells
摘要
Abstract
Objective To compare the three different methods for direct transfection of small exogenous nicotinamide adenine dinucleotide phosphate(NADPH)into cells by a non-metabolic pathway.Methods Three different transfec-tion reagents(X-tremeGENETM HP DNA,LipofectamineTM RNAiMAX and LipofectamineTM 2000)were used to find the transfection efficiency of NADPH into human osteosarcoma cell line U2OS and mouse embryonic fibroblast cell line 3T3L1.Their effects on adipocyte differentiation were compared by Oil Red O staining.Results Transfection of NADPH with X-tremeGENE HP DNA transfection reagent effectively increased intracellular NADPH level(P＜0.001).With the increase of NADPH transfection concentration(10 μmol/L NADPH and 10 μL transfection re-agent),the level of NADPH in cells increased in a dose-dependent manner.In addition,three transfection reagents were used to transfect NADPH in 3T3L1 preadipocytes.Only cells transfected with NADPH using X-tremeGENE HP DNA transfection reagent contained more lipids than control cells(P＜0.001).Conclusions Transfection of NADPH with X-tremeGENE HP DNA transfection reagent effectively increases the level of NADPH in cells.关键词
烟酰胺腺嘌呤二核苷酸磷酸(NADPH)/转染/细胞培养/脂肪细胞分化Key words
nicotinamide adenine dinucleotide phosphate(NADPH)/transfection/cell culture/adipocyte differentiation分类
生物科学引用本文复制引用
李皓玥,杜文静,李薇..利用不同方法将小分子NADPH转染进入细胞的比较[J].基础医学与临床,2024,44(5):613-618,6.基金项目
国家重点研发计划(2022YFA0806302,2019YFA0802600) （2022YFA0806302,2019YFA0802600）
细胞生态海河实验室项目(22HHXBSS0011) （22HHXBSS0011）
中国医学科学院医学与健康科技创新工程(2021-I2M-1-016) （2021-I2M-1-016）
