褪黑素抑制H2O2诱导的人脐静脉内皮细胞氧化应激及凋亡标志蛋白质的表达OACSTPCD

Objective To investigate the effects of melatonin(MT)on oxidative stress and expression of apoptosis marker protein in human umbilical vein endothelial cells(HUVECs)induced by hydrogen peroxide(H2O2).Methods HUVECs were divided into blank group and H2O2(100,200,300,400,600,700 mmol/L)groups.CCK8 method was used to select the best concentration for modeling.To clarify the effect of melatonin on oxidative stress injury caused by H2O2,HUVECs were divided into control group,H2O2 group and melatonin group.Western blot was used to detect the expression of p-p65/p65,SOD2 and cleaved-caspase 3.The activity of super-oxide dis-mutase(SOD),the concentration of malondialdehyde(MDA)and catalase(CAT)were measred by commercially available kits.Reactive oxygen species(ROS)staining was used to detect ROS content in HUVECs.Results H2O2 increased the expression of p-p65/p65 and cleaved-caspase 3(P＜0.001),decreased the expression of SOD2(P＜0.05)its biological activity(P＜0.001)and the concentration of CAT(P＜0.01),elevated the concentration of MDA(P＜0.001)and the level of ROS(P＜0.001)in HUVECs.Compared with H2O2 group,MT treatment de-creased the expression of p-p65/p65(P＜0.001),SOD2(P＜0.001)and cleaved-caspase 3(P＜0.01),increased the expression of SOD2(P＜0.001)as well as biological activity of SOD(P＜0.001)and the concentration of CAT(P＜0.05)reduced the concentration of MDA(P＜0.001)and the level of ROS(P＜0.001)in HUVECs.Conclu-sions Melatonin plays a protective role in oxidative stress injury of HUVECs induced by H2O2 through up-regula-ting the expression of SOD2 and down-regulating the expression of p-p65/p65 and cleaved-caspase 3.