油橄榄果渣熊果酸对沙门氏菌抑菌机理研究OACSTPCD

Using olive pomace ursolic acid as the material and Salmonella as the test organism,the growth curve,culture fluid conductivity,nucleic acid content,intracellular adenosine triphosphate(ATP)content changes and bacterial electron microscopy scanning methods to explore the inhibition mechanism of ursolic acid on Salmonella.The results showed that the cell membranes of Salmonella spp.treated with ursolic acid from olive pomace were disrupted and their growth rate was inhibited at all times,and the conductivity and nucleic acid content of the culture broth increased significantly and the ATP content decreased.Electron microscopy scans showed that ursolic acid caused the bacterium to crumple and sink inward,developing holes and disrup-ting the integrity of the cell membrane,causing leakage of intracellular material.This suggests that ursolic acid from olive pomace inhibits the growth of Salmonella and the mechanism of action is to alter the permeabili-ty of the Salmonella cell membrane and disrupt the morphological structure of the bacteriophage.