棉花学报2024，Vol.36Issue(1)：28-38,11.DOI:10.11963/cs20230044
棉花CMS-D2和CMS-D8不育系线粒体全基因组比较分析
Comparative analysis of whole mitochondrial genomes between CMS-D2 and CMS-D8 sterile lines in cotton
摘要
Abstract
[Objective]This research aims to investigate the sequence structural differences between the mitochondrial genomes of Gossypium harknessii sterile cytoplasm(CMS-D2)and G.trilobum sterile cytoplasm(CMS-D8),which can establish a foundation for the screening and identification of sterility-related genes.[Methods]Based on the sequencing and assembled results of the mitochondrial genome of D2A and D8A,the Synteny and Rearrangement Identifier(SyRI)software was used to identify structural variations,and recombination variation sites containing collinear and non-collinear regions were visualized with Plotsr.The annotation results of the D8A mitochondrial genome were utilized as a reference,and the amino acid sequences encoded by the open reading frame(ORF)annotated in the D2A mitochondrial genome were used for tblastn comparison to screen out the unique ORF in the D2 A mitochondrial genome.Subsequently,polymerase chain reaction(PCR)verification,relative expression level analysis,and bioinformatics analysis were performed.[Results]The mitochondrial genomes of the sterile lines D2A and D8A had two partially overlapped and adjacent inverted translocation regions.Seventeen specific ORF were found in D2A mitochondrial genome,and PCR verified the presence of 6 specific ORF(orf114e,orf121b-1,orf121b-2,orf138b-2,orf186a-2,and orf317a-2)in D2A.The relative expression levels of orf121b-1 and orf121b-2 were higher in 3-4 mm flower buds.orf114e,orf186a-2,and orf317a-2 have typical transmembrane domains and chimeric gene structures,which are consistent with some characteristics of sterility genes.[Conclusion]Two adjacent inverted translocation regions were identified between the mitochondrial genomes of D2A and D8A.There are six specific ORF in D2A,among which orf114e,orf186a-2,and orf317a-2 may be potentially related to sporophytic abortion of CMS-D2.关键词
棉花/细胞质雄性不育/线粒体基因组/孢子体不育/配子体不育/开放阅读框Key words
cotton/cytoplasmic male sterility/mitochondrial genome/sporophyte sterility/gametophyte sterility/open reading frame引用本文复制引用
葛李爽,邢朝柱,吴建勇,冯娟娟,张梦,郭立平,戚廷香,张学贤,李永旗,唐会妮,乔秀琴..棉花CMS-D2和CMS-D8不育系线粒体全基因组比较分析[J].棉花学报,2024,36(1):28-38,11.基金项目
河南省自然科学基金面上项目"orf610a调控棉花细胞质雄性不育的分子机制研究"(232300421168) （232300421168）
