非生物腐殖化的微生物生理效应和产物转化OA北大核心CSTPCD
Microbial Physiological Effects and Product Transformation of Abiotic Humification
制革废水中的可溶性有机物在生物处理系统的转化行为还不清楚.以单宁酸、甘氨酸和葡萄糖为原料模拟废水发生非生物腐殖化,并结合多元光谱技术,探究了非生物腐殖化产物在微生物纯培养和混合培养中的转化及对生物代谢活性的影响.结果表明:单宁酸能促进非生物腐殖化进程,同时经非生物腐殖化体系暴露后,纯培养在48 h-1 000 mg/L氨基酸类区域的荧光强度增强;混合培养在96 h-2 000 mg/L类腐殖酸区荧光强度最强,并新增了类溶解性微生物副产物的荧光区.衡量腐殖化程度的SUVA254值分析显示,混合培养的SUVA254值是纯培养的3倍,说明非生物腐殖化产物经混合培养转化后芳香性显著提升.研究结果揭示了非生物腐殖化和其生物转化产物是制约制革废水达标处理的重要控制因素.
The transformation behavior of dissolved organic matter in tannery wastewater is still unclear in biological treatment systems.Herein,tannic acid,glycine and glucose were used as raw materials to simulate the abiotic humification of wastewater.By combining with multivariate spectroscopy,we investigated the characteristics of the abiotic humus products,the transformation and the effects on the metabolic activity of microorganisms in both pure and mixed culture systems.Results showed that tannic acid promoted the abiotic humification process,while the fluorescence intensity of the amino acid-like region was enhanced at 48 h-1 000 mg/L in the pure culture after the microorganisms were exposed to the abiotic humification system.The fluorescence intensity was the strongest in the humic acid-like region at 96 h-2 000 mg/L in the mixed culture,and the fluorescence region of the solubilized microbial by-product-like products was added.Analysis of SUVA254,a measure of the degree of humification,showed that the mixed culture was three times higher than the pure culture,indicating a significant increase in the aromaticity of the transformed abiotic humic products compared to the mixed culture.This work revealed that abiotic humification and its biotransformation products were important control factors governing the standardized treatment of tannery wastewater.
朱超;周明芳;利浩男;马宏瑞;郝永永
陕西科技大学环境科学与工程学院,陕西西安 710021陕西科技大学轻工科学与工程学院,陕西西安 710021
环境科学
溶解性有机物非生物腐殖化大肠杆菌活性污泥
dissolved organic matterabiotic humificationEscherichia coliactivated sludge
《皮革科学与工程》 2024 (003)
17-24 / 8
国家水污染防治重大科技计划项目(2017ZX07602-001);国家自然科学基金项目(22076113)
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