中国海洋大学学报（自然科学版）2024，Vol.54Issue(5)：63-70,8.DOI:10.16441/j.cnki.hdxb.20220180
一株溶藻菌Zobellella sp.B307对太平洋亚历山大藻的溶藻特性及作用机制研究
The Algicidal Characteristics and Mechanism of an Algicidal Bacterium Zobel lella sp.B307 on Alexanderium pacificum
摘要
Abstract
In order to study the algicidal mechanism of algicidal bacteria on red tide dinoflagellate,a salt-tolerant bacterium Zobellella sp.B307 with algicidal activity to the Alexandrium pacificum was isolated from the sediments of Jiaozhou Bay.To explore the algicidal mechanism of Zobellella sp.B307,we investigated its effect on the cellular structure,physiologyl and molecular mechanism of A.pacifi-cum.The results showed that the inhibition mechanism of the strain was indirect lytic effects causing by extracellular active substances.And after 72 h cultivation with bacterium addition,the algicidal rate reached up to 91%,revealing its strong algicidal activity against A.pacificum.Meanwhile,we ob-served cell wall damage,chlorophyll-a and total protein contents decrease,and the excessive production of reactive oxygen species(ROS),superoxide dismutase(SOD)and catalase(CAT).It indicated that the serious oxidative damage caused by algae-lysing substances was the direct cause of A.pacificum death.The expression level of heat shock protein(HSP)gene was significantly upregulated,suggesting that algae-lysing substances could induce the production of HSP,and the combined effects of highly ex-pressed HSP and highly activited antioxidant system of A.pacificum could eliminate ROS and alleviate the algae cells damage caused by oxidative stress.In addition,the expression of A.pacificum clathrin gene was significantly upregulated,indicating algae cells could enhanced heterotrophic function to com-pensate the loss of photosynthesis caused by algae-lysing substances.The results of this study will con-tribute to explain the newly founded relationship between bacteria and algae,and explore the new bio-logical methods of red tide control.关键词
赤潮/亚历山大藻/溶藻细菌/溶藻机理/基因表达Key words
red tide/Alexandrium sp./algicidal bacteria/algicidal mechanism/gene expression分类
资源环境引用本文复制引用
马双慧,白洁,孙鹏飞,王登宇,相壮壮,李辉,晨曦..一株溶藻菌Zobellella sp.B307对太平洋亚历山大藻的溶藻特性及作用机制研究[J].中国海洋大学学报（自然科学版）,2024,54(5):63-70,8.基金项目
自然资源部热带海洋生态系统与生物资源重点实验室开放基金课题项目(2021QN07)资助 Supported by the Fund of the Key Laboratory of Tropical Marine Ecosystem and Bioresources,Ministry of Natural Resources(2021QN07) （2021QN07）
