食品工业科技2024，Vol.45Issue(9)：26-35,10.DOI:10.13386/j.issn1002-0306.2023070261
基于转录组学探讨林蛙油对卵泡发育障碍大鼠的影响
Transcriptomics-based Exploration of the Effects of Oviductus Ranae on Rats with Impaired Follicular Development
摘要
Abstract
Objective:The effects of Oviductus Ranae(OR)on cyclophosphamide-induced follicular developmental disorders were analyzed from the perspective of transcriptomics,which provided sufficient theoretical and experimental basis for the study of estrogen-like mechanism of OR.Methods:Sixty screened female Wistar rats were randomly divided into control group(C),model group(M),positive group(P),OR low-dose(ORL)and high-dose group(ORH).Except for the control group,all rats were injected with cyclophosphamide intraperitoneally to establish the rat model of follicular development dysfunction.Except for the model group,the other rats were given corresponding drugs by gavage,and the serum sex hormone contents were determined after 14～16 days of continuous gavage.The ovaries and uterus were extracted and weighed for recording and calculating the organ index.One ovary and uterus were stained with HE staining to calculate the number of follicles at all levels and the thickness of the endometrium,while the other ovary was analyzed by transcriptome,and the differentially expressed genes were screened and analyzed by GO and KEGG enrichment analyses.Results:The results showed that the rat follicular development dysfunction model was successfully established.Compared with the rats in the M,the wet weight and index of the uterus of rats in the ORL and ORH significantly increased(P＜0.05),and the contents of P and E2 significantly increased(P＜0.05).The number of preantral follicles and corpus luteum of rats in the ORL and ORH increased significantly(P＜0.05),and the thickness of endometrium increased significantly(P＜0.01),with statistically significant differences,and the number of antral follicles in the ORL increased significantly(P＜0.05).The transcriptome results showed that there were 738 and 572 differential genes between the ORL,ORH and M,respectively.GO enrichment analysis enriched 1790 and 1616 entries and the KEGG enrichment analysis was mainly focused on the PI3K/Akt signaling pathway and the B-cell receptor signaling pathway.Conclusion:OR can effectively improve serum sex hormone levels,ovarian follicular development,and endometrial atrophy in rats with cyclophosphamide-induced follicular dysfunction,and its molecular mechanism may be related to pathways and genes such as PI3K/Akt and B-cell receptor.关键词
林蛙油/卵泡发育障碍/卵巢/环磷酰胺/转录组Key words
Oviductus Ranae(OR)/follicular developmental dysfunction/ovary/cyclophosphamide/transcriptome分类
医药卫生引用本文复制引用
何星瑶,王玉,王君淑,王晓汇,邸琳,刘新宇,赵宏宇,张凤清..基于转录组学探讨林蛙油对卵泡发育障碍大鼠的影响[J].食品工业科技,2024,45(9):26-35,10.基金项目
吉林省自然科学基金(YDZJ202201ZYTS264) （YDZJ202201ZYTS264）
长春市科技发展计划项目(21ZY62). （21ZY62）
