食品工业科技2024，Vol.45Issue(9)：196-204,9.DOI:10.13386/j.issn1002-0306.2023070139
响应面法优化准噶尔山楂总三萜提取工艺及其纯化工艺研究
Optimization of Extraction Process of Total Triterpenoids from Crataegus songarica by Response Surface Methodology and Its Purification Process
摘要
Abstract
In order to improve the high-value utilization of Crataegus songarica,the single factor experiment,orthogonal experiment and response surface design were used to optimize the extraction process of total triterpenes from Crataegus songarica by compound enzyme assisted extraction,and the purification process was optimized.The results showed that the optimal extraction process of total triterpenes from Crataegus songarica was as follows:The addition amount of cellulase,pectinase and papain was 4％,4％and 2％,respectively,the ratio of material to liquid was 1∶24 g/mL,the temperature was 51 ℃,the pH was 4.5,and the enzymolysis time was 76 min.Under these conditions,the extraction amount of total triterpenes from Crataegus songarica was(36.570±0.332)mg/g,and the extraction amount of total triterpenes was(53.782±0.673)mg/g by ultrasonic extraction again.The purification process was as follows:The concentration of the sample solution was 6 mg/mL,the flow rate was 1 mL/min,the sample volume was 15 mL,the elution condition was 50 mL,and 70％ethanol.The purity of the total triterpenoids was 29.93％,and the recovery rate was 81.25％.The above results indicate that the compound enzymatic method is feasible and stable for the extraction and purification of total triterpenoids from Crataegus songarica by macroporous resin.关键词
准噶尔山楂/三萜/酶辅助提取/大孔树脂/纯化Key words
Crataegus songarica/triterpenes/enzyme-assisted extraction/macroporous resin/purification分类
轻工纺织引用本文复制引用
冯琳,罗世博,古丽格娜·皮达买买提,刘媛梦,高红艳..响应面法优化准噶尔山楂总三萜提取工艺及其纯化工艺研究[J].食品工业科技,2024,45(9):196-204,9.基金项目
新疆普通高等学校天然产物化学与应用重点实验室开放课题(2023YSHXYB01). （2023YSHXYB01）
