食品工业科技2024，Vol.45Issue(9)：255-264,10.DOI:10.13386/j.issn1002-0306.2023050213
QuEChERS-超高效液相色谱-串联质谱法测定茶叶中47种农药残留
Determination of 47 Pesticide Residues in Tea by QuEChERS Method Combined with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
An analysis method for simultaneous determination of 47 kinds of pesticide residues in tea was established by QuEChERS method combined with ultra performance liquid chromatography-tandem mass spectrometry.The samples were extracted by acetonitrile containing 1％acetic acid,and purified by QuEChERS method.The 47 pesticides were determined by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)with an electrospray ion source(ESI±)in positive and negative ion switching multiple reaction monitoring(MRM),and quantified by matrix-matched external standard method.Method validation revealed satisfactory linearities with correlation coefficients(r)between 0.9971 and 0.9996,and limits of detection were 0.001～0.006 mg/kg while the limits of quantitation were 0.002～0.020 mg/kg,the average recoveries at three spiked levels were in the range of 73.4％～114.3％,with relative standard deviations(n=6)of 0.3％～19.9％.The method is sensitive,accurate,stable,and can meet the requirements for determination of 47 pesticide residues in tea.关键词
QuEChERS/茶叶/农药残留/超高效液相色谱-串联质谱法(UPLC-MS/MS)Key words
QuEChERS/tea/pesticide residues/ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)分类
轻工纺织引用本文复制引用
刘华文,邓美晴,陈北燕..QuEChERS-超高效液相色谱-串联质谱法测定茶叶中47种农药残留[J].食品工业科技,2024,45(9):255-264,10.基金项目
植物源性食品中植物生长调节剂风险筛查研究(GXSJKJ2023-18). （GXSJKJ2023-18）
