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微酸性电解水对空肠弯曲菌的杀灭特性及机制

宋欣坤 庞柳欣 牛玲玲 岳婷 牛际涵 张春玲

食品科学2024,Vol.45Issue(8):29-36,8.
食品科学2024,Vol.45Issue(8):29-36,8.DOI:10.7506/spkx1002-6630-20230619-147

微酸性电解水对空肠弯曲菌的杀灭特性及机制

Inactivation Characteristics and Mechanism of Slightly Acidic Electrolyzed Water on Campylobacter jejuni

宋欣坤 1庞柳欣 1牛玲玲 1岳婷 1牛际涵 1张春玲1

作者信息

  • 1. 西北农林科技大学食品科学与工程学院,陕西 杨凌 712100
  • 折叠

摘要

Abstract

In this study,the bactericidal activity and mechanism of slightly acidic electrolyzed water(SAEW)against Campylobacter jejuni were examined.The effect of available chlorine concentration(ACC),treatment time and protein addition on the bactericidal activity of SAEW were investigated.In addition,the killing effect of SAEW on multi-generational cultures of C.jejuni was analyzed.The bactericidal mechanism was explored based on the changes in cell membrane integrity,morphology,and contents.The results showed that the inactivation of C.jejuni by SAEW was positively correlated with ACC and treatment time.Exogenous protein significantly weakened the bactericidal efficacy of SAEW.C.jejuni did not develop resistance to SAEW within ten passages.SAEW could destroy the integrity of the cell membrane.After treatment with SAEW,the cell surface was sunken,wrinkled and broken,and nucleic acid,protein and ATP leaked out from the cells,indicating that cell membrane damage may be the major reason for SAEW to inactivate C.jejuni.

关键词

微酸性电解水/空肠弯曲菌/蛋白质/杀灭特性/机制

Key words

slightly acidic electrolyzed water/Campylobacter jejuni/protein/killing characteristics/mechanism

分类

轻工业

引用本文复制引用

宋欣坤,庞柳欣,牛玲玲,岳婷,牛际涵,张春玲..微酸性电解水对空肠弯曲菌的杀灭特性及机制[J].食品科学,2024,45(8):29-36,8.

基金项目

国家自然科学基金青年科学基金项目(31801658) (31801658)

食品科学

OA北大核心CSTPCD

1002-6630

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