食品科学2024，Vol.45Issue(8)：105-113,9.DOI:10.7506/spkx1002-6630-20230628-225
金针菇多糖通过调节髓系细胞生成缓解苯并[a]芘染毒小鼠血管炎症
Flammulina velutipes Polysaccharides Alleviate Vascular Inflammation in Mice Exposed to Benzo[a]pyrene via Regulating Myeloid Cytogenesis
摘要
Abstract
Flammulina velutipes polysaccharides(FVP)have been reported to exhibit excellent anti-inflammatory and antioxidant effects.This study further investigated the efficacy and mechanism of action of FVP in alleviating vascular injury induced by benzo[a]pyrene(BaP)in mice.The expression of vascular inflammatory cytokines was assessed through immunohistochemistry analysis.Flow cytometry was used to analyze the proportion of pro-inflammatory cells in the peripheral blood and spleen as well as the composition of hematopoietic stem cell(HSC)subsets in bone marrow.The results demonstrated that FVP treatment mitigated the expression of vascular pro-inflammatory cytokines such as interleukin-6,monocyte chemoattractant protein-1 and intercellular cell adhesion molecule-1 in mice induced by Ba P.Moreover,FVP treatment significantly inhibited the increases in the proportions of monocytes,macrophages and neutrophils in the peripheral blood and those of macrophages and neutrophils in the spleen of BaP-induced mice.Further analysis of bone marrow HSC subsets revealed that BaP exposure elevated the proportions of hematopoietic progenitor cells and granulocyte-macrophage progenitor cells in mouse bone marrow,while reducing the proportions of hematopoietic stem cells and common myeloid progenitor cells,which were reversed by FVP treatment,thus restoring bone marrow HSC subsets to the level of the healthy control group.At the cellular level,FVP suppressed BaP-induced oxidative stress in Raw264.7 cells.Taken together,FVP alleviate Ba P-induced vascular inflammatory injury by maintaining the homeostasis of the bone marrow hematopoietic system,thereby regulating the output of pro-inflammatory cells into the circulatory system,and down-regulating the proportion of peripheral pro-inflammatory cells.关键词
金针菇多糖/苯并[a]芘/血管炎症/造血作用/髓系细胞生成Key words
Flammulina velutipes polysaccharides/benzo[a]pyrene/vascular inflammation/hemopoiesis/myeloid cell generation分类
轻工纺织引用本文复制引用
牛泽淼,李鑫,陈宁,梁振华,赵娟,吕品,张岩,付程浩..金针菇多糖通过调节髓系细胞生成缓解苯并[a]芘染毒小鼠血管炎症[J].食品科学,2024,45(8):105-113,9.基金项目
国家自然科学基金面上项目(32272328) （32272328）
河北医科大学研究生创新资助项目(XCXZZS202310) （XCXZZS202310）
河北省教育厅青年科学基金项目(QN2023229) （QN2023229）
河北省人社厅择优资助博士后科研项目(B2022003031) （B2022003031）
