食品科学2024，Vol.45Issue(8)：145-152,8.DOI:10.7506/spkx1002-6630-20230708-074
荔枝落果中A型低聚原花青素抑制类甜蛋白促炎机制
Inhibitory Mechanism of A-type Oligomeric Proanthocyanidins from Abscising Litchi Fruits on Proinflammation Induced by Litchi Thaumatin-Like Protein
摘要
Abstract
This study investigated the mechanism by which A-type oligomeric proanthocyanidins(A-OPC)from abscising litchi fruits inhibit proinflammation induced by litchi thaumatin-like protein(LcTLP).A-OPC were prepared by sequential ethanol extraction and purification by macroporous adsorption resin and preparative liquid chromatography.Structural analysis showed that A-OPC were mainly composed of proanthocyanidin A2,two A-type proanthocyanidin trimers and one A-type tetramer,with a purity of 88.69%.At 120 μg/mL concentration,A-OPC inhibited the secretion of nitric oxide(NO),interleukin(IL)-6 and tumor necrosis factor-α(TNF-α)by 58.38%,39.80%and 86.31%in RAW264.7 cells treated with LcTLP,respectively(P<0.01).Western blot showed that 120 μg/mL A-OPC reduced the expression of inducible nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2),and significantly decreased the phosphorylation levels of p65,inhibitor of NF-κB(IκBα),c-Jun N-terminal kinase(JNK),extracellular signal-regulated kinase(ERK)and p38.Therefore,A-OPC down-regulated the phosphorylation levels of downstream proteins in the nuclear transcription factor-κB(NF-κB)and mitogen-activated protein kinase(MAPK)signaling pathways,thereby suppressing LcTLP-induced inflammatory response.关键词
荔枝落果/A型低聚原花青素/荔枝类甜蛋白/抗炎机制Key words
litchi fruit/A-type oligomeric proanthocyanidins/litchi thaumatin-like protein/anti-inflammatory mechanism分类
轻工纺织引用本文复制引用
罗杨合,曾诗蔼,李洛欣,谢翀,闫景坤,李玉婷,罗英捷,赵雷..荔枝落果中A型低聚原花青素抑制类甜蛋白促炎机制[J].食品科学,2024,45(8):145-152,8.基金项目
广西康养食品科学与技术重点实验室开放课题(GXKYSYS202204) （GXKYSYS202204）
东莞市重点领域研发项目(20221200300082) （20221200300082）
广东省自然科学基金项目(2023A1515012599) （2023A1515012599）
广州市科技计划项目(2023B01J2004) （2023B01J2004）
"十四五"广东省农业科技创新十大主攻方向"揭榜挂帅"项目(2023SDZG04) （2023SDZG04）
