食品科学2024，Vol.45Issue(8)：248-256,9.DOI:10.7506/spkx1002-6630-20230602-009
茉莉酸甲酯类生长调节剂对'蛇龙珠'葡萄果实品质及脂氧合酶代谢途径的影响
Effects of the Plant Growth Regulator Methyl Jasmonate on Fruit Quality and Lipoxygenase Metabolic Pathway of Cabernet Gernischt
摘要
Abstract
In this study,the optimal concentrations of methyl jasmonate(MeJA)and methyl dihydrojasmonate(MDJA)for Cabernet Gernischt were selected based on basic physicochemical properties of grapes.The key volatile metabolites and key enzyme activities in response to MeJA and MDJA were analyzed by headspace solid-phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS)and enzyme-linked immunosorbent assay(ELISA),respectively.The effects of pre-harvest MeJA or MDJA treatment on the fruit quality and green leaf volatile components in the lipoxygenase(LOX)metabolic pathway of Cabernet Gernischt were also investigated.The findings revealed that the application of 0.1 mmol/L MeJA or 5 mmol/L MDJA significantly enhanced mass of 100 berries,total sugar content,soluble solids content,pH,total phenol and total anthocyanin contents.Compared with the control group,MeJA increased these indexes by 10.24%,27.83%,28.24%,4.80%,77.63%and 85.29%,respectively,while MDJA increased them by 9.15%,24.01%,24.41%,8.30%,69.21%and 71.57%,respectively.Furthermore,MeJA and MDJA reduced titratable acid content by 40.0%and 30.24%,respectively.Accordingly,both MeJA and MDJA significantly improved fruit quality.Moreover,they significantly increased the contents of C6 compounds responsible for the typical aroma of Cabernet Gernischet.Significant correlations were observed between the activity of each key enzyme in the lipoxygenase metabolic pathway and the corresponding metabolites,indicating that the application of MeJA or MDJA at a suitable concentration could positively influence the accumulation of green leaf volatiles in Cabernet Gernischt.关键词
'蛇龙珠'/茉莉酸甲酯/二氢茉莉酸甲酯/葡萄品质/脂氧合酶代谢Key words
Cabernet Gernischt/methyl jasmonate/methyl dihydrojasmonate/grape quality/lipoxygenase metabolism分类
农业科技引用本文复制引用
冶楠,张珍,吕转转,杜建明,张生祥,李蔚..茉莉酸甲酯类生长调节剂对'蛇龙珠'葡萄果实品质及脂氧合酶代谢途径的影响[J].食品科学,2024,45(8):248-256,9.基金项目
甘肃农业大学公招博士科研启动基金项目(GAU-KYQD-2020-21) （GAU-KYQD-2020-21）
甘肃省青年科技基金计划项目(21JR7RA849) （21JR7RA849）
