食品科学2024，Vol.45Issue(8)：257-264,8.DOI:10.7506/spkx1002-6630-20230705-040
通过式固相萃取-超高效液相色谱-串联质谱法测定食品中的氯米芬、曲美他嗪和美度铵
Simultaneous Determination of Clomiphene,Trimetazidine and Meldonium in Foods by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry Combined with Pass-Through Solid Phase Extraction
摘要
Abstract
In this study,we established a method for the determination of three metabolic regulators including clomiphene,trimetazidine and meldonium in foods by pass-through solid phase extraction combined with ultra-high performance liquid chromatography-tandem mass spectrometry(SPE-UPLC-MS/MS).The samples were extracted with 5%formic acid in acetonitrile,and the extract was purified on a pass-through solid phase extraction column and chromatographically separated on an Amide column(3.0 mm×100 mm,1.7 μm).The separated analytes were detected in the multiple reaction monitoring(MRM)mode,and quantitated by an internal standard method.Good linearity was observed for clomiphene and trimetazidine within the concentration range of 1.0-40.0 ng/mL,and 5.0-200.0 ng/mL for meldonium.The average recoveries for spiked samples ranged from 81.3%to 100.7%,and the relative standard deviations(RSD)were between 0.3%and 6.1%(n = 6).The developed method was simple,rapid and practical with low matrix interferences,and was generally suitable for the determination of clomiphene,trimetazidine and meldonium in foods,which could provide effective technical support for the assurance of food safety for sports events.关键词
氯米芬/曲美他嗪/美度铵/超高效液相色谱-串联质谱法/固相萃取Key words
clomiphene/trimetazidine/meldonium/ultra-high performance liquid chromatography-tandem mass spectrometry/solid phase extraction分类
化学化工引用本文复制引用
宋娟,李君,张阳阳,姚蕾珺,肖全伟,李绍波,戴琴..通过式固相萃取-超高效液相色谱-串联质谱法测定食品中的氯米芬、曲美他嗪和美度铵[J].食品科学,2024,45(8):257-264,8.基金项目
"十四五"国家重点研发计划重点专项(2022YFF0710405) （2022YFF0710405）
国家市场监督管理总局项目(2021YJ009) （2021YJ009）
四川省科技计划项目(2022YFS0511) （2022YFS0511）
