食品科学2024，Vol.45Issue(8)：265-271,7.DOI:10.7506/spkx1002-6630-20230712-153
高效液相色谱法测定婴幼儿配方奶粉中1,3-二油酸-2-棕榈酸甘油三酯
Determination of 1,3-Dioleic acid-2-palmitoyl triglyceride in Infant Formula by High Performance Liquid Chromatography
摘要
Abstract
A method for the quantification of 1,3-dioleyl-2-palmitoyl-glycerol(OPO)in infant formula was developed.The samples were treated with ammonia and extracted with organic solvents.The fat containing OPO was purified on a NH2 solid-phase extraction(SPE)cartridge packed with aminopropyl as the sorbent.The eluate was separated by silver ion chromatography using 0.55%acetonitrile-hexane as the mobile phase.The detection was carried out with a high performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD).This novel procedure enabled the complete separation of OPO and its isomer 1,2-dioleyl-3-palmitoyl-glycerol(OOP),thus allowing for the accurate quantification of OPO.The developed method showed the desired linearity in the concentration range of 25-500 μg/mL with a determination coefficient(R2)of 0.999 6.The limits of detection(LOD)and limits of quantification(LOQ)were 0.30 and 0.90 g/kg,respectively.At spiked concentrations from 1 to 96 g/kg,the average recoveries of OPO varied from 97.1%to 104.2%with relative standard deviations(RSD)between 1.2%and 2.9%.The precision and accuracy of this method met the relevant requirements,and it passed the inter-laboratory collaborative validation.Our investigation analyzed 39 commercial samples of OPO-fortified infant formula in China,revealing that the measured OPO content only accounted for 28.4%to 59.7%of the labelled value,which is mainly due to the inconsistency of detection methods.关键词
高效液相色谱/1,3-二油酸-2-棕榈酸甘油三酯/婴幼儿配方奶粉Key words
high performance liquid chromatography/1,3-dioleic acid-2-palmitoyl triglyceride/infant formula分类
轻工业引用本文复制引用
戴嵘德,刘峰,傅武胜,黄思婷,杨贵芝,刘文菁..高效液相色谱法测定婴幼儿配方奶粉中1,3-二油酸-2-棕榈酸甘油三酯[J].食品科学,2024,45(8):265-271,7.基金项目
卫生部食品安全国家标准制定项目(spaq-2012-71) （spaq-2012-71）
