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不同稻虾模式对硫酸盐还原菌群落结构及多样性的影响OA北大核心CSTPCD

Effects of different rice-crayfish models on community structure and diversity of sulfate-reducing bacteria

中文摘要英文摘要

硫酸盐还原菌(SRB)作为一种土壤中普遍存在的微生物,在生物地球化学循环中发挥关键作用.本研究以常规水稻单作模式为对照(CK),无环沟式稻虾(水稻和克氏原螯虾)共作模式(RS0)、有环沟式稻虾轮作模式(RS1)与有环沟式稻虾共作模式(RS2)为研究对象,采用16SrDNA高通量测序技术,探究不同稻虾模式对土壤硫酸盐还原菌(SRB)群落结构及多样性的影响.结果表明:与CK相比,3种稻虾模式均显著降低了水稻成熟期土壤氧化还原电位(Eh)和硫化物、硫酸盐、全硫含量,提高了pH和有机质、速效钾、碱解氮含量,其中RS0提升效果最为显著;3种稻虾模式SRB群落结构存在差异:RS1、RS2和RS0处理的δ-变形菌纲相对丰度比CK分别下降46.00%、63.61%和51.94%,而α-变形菌纲则分别提高402.52%、441.01%和584.17%.3种稻虾模式硫酸盐还原菌Observed_species指数、Shannon指数和Simpson指数与CK相比均显著提高,但不同稻虾模式间丰富度及多样性没有显著差异.RDA分析表明,速效钾、Eh、有机质、有效磷、碱解氮、硫酸盐、硫化物、全硫和pH是影响稻田土壤SRB群落结构的影响因子,其中全硫和硫化物是主要因子.综上,稻虾模式能维持或者提高稻田土壤养分状况和SRB群落丰富度及多样性,改善稻田土壤SRB群落结构,结果可为稻虾种养土壤生态健康及稻田综合种养土壤微生物研究提供参考和支撑.

The rice-crayfish integrated farming model,an ecological agricultural system,combines rice cultivation with the co-culture or rotation of crayfish(Procambarus clarkii),forming a mutually beneficial symbiosis.Sulfate-reducing bacteria(SRB)are widespread soil microbes that are pivotal in driving biogeochemical cycles.Researches on SRB is mainly centered on traditional rice fields,with comparatively few studies addressing their role in innovative rice-crayfish composite ecological agriculture systems.This study compared traditional rice monoculture(CK)with three distinct rice-crayfish systems:integrated rice-crayfish system without ring groove(RS0),integrated rice-crayfish rotation system with ring grooves(RS1),and integrated rice-crayfish system with ring grooves(RS2).Using 16S rDNA high-throughput sequencing technology,this study explored the effect of different rice-crayfish mod-els on the community structure and diversity of soil SRB.Compared to the traditional rice monoculture,all three rice-crayfish models significantly decreased the soil oxidation-reduction potential(Eh)and contents of sulfide,sulfate,and total sulfur at the rice maturity stage.There was also a significantly increase in soil pH,and contents of organic matter,available potassium and nitrogen,with the most significant improvement observed in the RS0 model.This study revealed distinct SRB community structures across the three rice-crayfish models.Compared to the traditional rice monoculture,the δ-Proteobacteria class exhibited a decrease in relative abundance by 46.00%,63.61%,and 51.94%in the RS1,RS2,and RS0 models,respectively,while the α-Proteobacteria class showed a substantial increase of 402.52%,441.01%,and 584.17%,respectively.The Observed_species index,Shannon index,and Simpson index for SRB in all three rice-crayfish models were significantly higher than those in the CK,but there were no significant differences in richness and diversity among different rice-crayfish models.Redundancy analysis(RDA)revealed that factors such as available potassium,Eh,organic matter,available phosphorus,available nitrogen,sulfate,sulfide,total sulfur,and pH influenced the SRB community struc-ture of rice field soil,with total sulfur and sulfide being the primary factors.In summary,the rice-crayfish model demonstrated the ca-pacity to either maintain or improve the nutrient status,richness,and diversity of SRB communities in paddy soil,thereby effectively enhancing the structure of the SRB community in paddy fields.These findings offer valuable insights for supporting research on soil ecological health and soil microbiology of integrated rice-crayfish farming systems.

黎衍亮;邱秀文;江玉梅;倪才英;肖罗长;梁以豪;刘鑫

江西师范大学地理与环境学院/鄱阳湖湿地与流域研究教育部重点实验室 南昌 330022江苏农林职业技术学院风景园林学院 镇江 212499江西师范大学生命科学学院 南昌 330022

农业科学

稻虾种养轮作共作硫酸盐还原菌群落结构多样性

Rice-crayfish farmingCrop rotationCo-cultureSulfate reducting bacteriaCommunity structureDiversity

《中国生态农业学报(中英文)》 2024 (004)

559-570 / 12

国家自然科学基金项目(42167006)和江西省现代农业产业技术体系建设专项(JXARS-12-环境控制)资助 This study was supported by the National Natural Science Foundation of China(42167006)and Jiangxi Province Modern Agricultural Industrial Tech-nology System Construction Project(JXARS-12-Environmental Control).

10.12357/cjea.20230538

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