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亚紫裸伞的生物学特性及驯化栽培OA北大核心CSTPCD

Biological Characteristics,Domestication and Cultivation of Wild Gymnopilus subpurpuratus

中文摘要英文摘要

[目的]为实现西藏野生真菌资源的高效开发与利用,促进食药用真菌产业的发展.[方法]对采自西藏自治区林芝市波密县易贡乡的一株野生真菌进行分离纯化,依据形态学特征和ITS序列进行菌种鉴定,并对该菌株进行了生物学特性探究和驯化栽培试验.[结果]结合形态学特征和ITS系统发育树结果将该菌株鉴定为亚紫裸伞Gymnopilus subpurpuratus;生物学特性分析表明,亚紫裸伞菌丝生长最佳碳源为葡萄糖,最佳氮源为酵母粉,最适pH为 6.0,最适温度为 30℃;驯化结果表明,亚紫裸伞在以木屑为主要栽培料的培养基中的满袋时间为 39 d,原基形成时间为 18 d,出原基后经 8 d达到采收期,单朵鲜重 45.99 g.[结论]成功以木屑为主要栽培料获得亚紫裸伞的人工驯化子实体,增加了药用菌的人工驯化新种类.

[Objective]This work is to realize the efficient development and utilization of Xizang's wild fungi resources and promote the development and growth of edible and medicinal fungi industry.[Method]A wild fungus collected fromYigong township,Bomi county,Linzhi city,Xizang autonomous region was isolated and purified,and the strain was identified according to morphological characteristics and its sequence.[Result]Combined with morphological characteristics and ITS phylogenetic tree results,the strain was identified as Gymnopilus subpurpuratus.The results show that the best carbon source for mycelium growth is glucose,the best nitrogen source is yeast powder,the optimal pH is 6.0,and the optimal temperature is 30℃.The results of domestication show that the filling-bag time in the culture medium with sawdust as the main cultivation material is 39 d,the formation time of primordium is 18 d,the harvest time is 8 dafter primordium was out,and the fresh weight of single fungus is 45.99 g.[Conclusion]To sum up,the artificial domesticated fruiting body of G.subpurpuratus is successfully obtained by using sawdust as the main cultivation material,which increases the new species of artificial domestication of medicinal fungi.

饶永斌;张君丽

三明市农业农村局,三明 365000西藏自治区农牧科学院蔬菜研究所,拉萨 850000

亚紫裸伞鉴定生物学特性驯化栽培

Gymnopilus subpurpuratusidentificationbiological characteristicsdomestication and cultivation

《生物技术通报》 2024 (004)

264-270 / 7

特色食用菌种质资源创新研究与菌种繁育关键技术集成示范(XZ202001ZY0041N)

10.13560/j.cnki.biotech.bull.1985.2023-1040

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