烟草科技2024，Vol.57Issue(4)：42-52,11.DOI:10.16135/j.issn1002-0861.2023.0384
干旱胁迫下外源褪黑素对旺长期烤烟生理特性的影响
Effects of exogenous melatonin on physiological characteristics of drought-stressed,flue-cured tobacco at fast-growing stage
摘要
Abstract
To investigate the influence of exogenous melatonin on the physiological characteristics of flue-cured tobacco at fast-growing stage under drought stress,a pot experiment was conducted using the flue-cured tobacco cultivar K326 in southwestern tobacco growing areas.The physiological regulatory effects of root irrigation and foliar application of melatonin at different concentrations(0,0.05,0.10,0.15,0.20,0.40 mmol/L)on K326 under drought stress were investigated,and the indicators that could alleviate drought stress on K326 were screened using principal component analysis.Meanwhile,a comprehensive evaluation was carried out using the membership function method.The results indicated that drought stress caused a decrease in the biomass of cured tobacco and significantly inhibited plant growth.Exogenous melatonin effectively increased biomass under drought stress,increased relative leaf water content(RWC),chlorophyll content,superoxide dismutase(SOD)activity,peroxidase(POD)activity,catalase(CAT)activity,proline(Pro)and soluble protein(SP)content,while decreasing leaf malondialdehyde(MDA)content and superoxide anion(O2-)production rate of the flue-cured tobacco.Principal component analysis revealed that antioxidant factors had the highest proportion among the evaluated indicators for alleviation of drought stress by melatonin,followed by photosynthetic factors and osmotic regulation factors.Comprehensive evaluation using the membership function method showed that foliar application of 0.10 mmol/L melatonin solution gave the best results.Therefore,the application of exogenous melatonin can potentially alleviate the inhibitory effects of drought stress on the growth of flue-cured tobacco at the fast-growing stage and enhance its adaptability to drought conditions.关键词
烤烟/干旱胁迫/褪黑素/生理指标/主成分分析/综合评价Key words
Flue-cured tobacco/Drought stress/Melatonin/Physiological index/Principal component analysis/Comprehensive evaluation分类
农业科技引用本文复制引用
崔凯东,张雪,顾开元,郭金格,汪芬芬,刘铭,周玉玲,杨奇,谢小玉..干旱胁迫下外源褪黑素对旺长期烤烟生理特性的影响[J].烟草科技,2024,57(4):42-52,11.基金项目
云南省烟草公司大理州公司科技计划项目"烤冻多用房开发及配套应用模式研究"(DLYC2022006) （DLYC2022006）
重庆市大学生创新训练项目(S202310635219). （S202310635219）
