Obg样ATP酶1促进人B细胞淋巴瘤Romas细胞生长的机制研究OA北大核心CSTPCD

AIM:To explore the role and mechanisms of Obg-like ATPase 1(OLA1)gene in the growth of hu-man B-cell lymphoma Romas cells.METHODS:The Gene Expression Profiling Interactive Analysis(GEPIA)database was utilized to analyze the differential expression of OLA1 in diffuse large B-cell lymphoma(DLBCL)tissues,and the im-pact of OLA1 expression on the survival rate of patients was examined by the GSE181063 database.The stable cell lines with knockdown of the OLA1 gene(OLA1-sh)and negative control were established by Romas cells via infection with retro-virus.The growth of Romas cells were assessed by cell counting,CCK-8 assay,subcutaneous tumor experiment in mice and Ki67 immunohistochemical staining.Flow cytometry was employed to investigate the cell cycle and apoptosis.TUNEL fluorescence staining was conducted to evaluate the apoptosis.The relationship between OLA1 gene and cell cycle/apopto-sis-related genes was analyzed by differential genes from 48 DLBCL patients in The Cancer Genome Atlas(TCGA)data-base.RT-qPCR was utilized to detect the cell cycle-and apoptosis-related genes.Western blot analysis was performed to detect the cell cycle-and apoptosis-related proteins.RESULTS:The OLA1 gene was highly expressed in DLBCL(P<0.05),and OLA1 knockdown significantly inhibited Romas cell proliferation,arrested the cell cycle,and induced apopto-sis(P<0.05 or P<0.01).Additionally,OLA1 knockdown promoted the expression of Bax,and suppressed cyclin-depen-dent kinase 6(CDK6),cyclin E1 and BCL2 expression(P<0.05 or P<0.01).CONCLUSION:Knockdown of OLA1 markedly suppresses the growth of human B-cell lymphoma Romas cells.The underlying mechanism might involve the blockade of cell cycle and the induction of apoptosis.