中国病理生理杂志2024，Vol.40Issue(4)：610-618,9.DOI:10.3969/j.issn.1000-4718.2024.04.005
藏药七十味珍珠丸对帕金森病模型大鼠认知能力的影响
Effect of Tibetan medicine Ranasampel on cognitive performance in Par-kinson disease model rats
摘要
Abstract
AIM:To investigate the effect of Tibetan medicine Ranasampel(RNSP)on the cognitive ability of Parkinson disease(PD)model rats.METHODS:The PD rats were established by 6-hydroxydopamine(6-OHDA)single-point injection in right striatum.The animals were randomly divided into PD model group,Medopar group(Medopar 50 mg/kg),RNSP low-dose,medium-dose,high-dose group(90,180,360 mg/kg)and normal group,with 8 rats in each group.The rats in each group were intragastrically administered once a day for 4 weeks after modeling,while the PD mod-el group and normal group received equal volume of saline.The rats were weighed on first day of RNSP administration,then on the 7th,14th,21th and 28th days.After 4 weeks of RNSP administration,apomorphine induced Rotation experi-ment and the Morris water maze experiment were performed.The number of tyrosine hydroxylase(TH)positive neurons in the substantia nigra densities(SNc)and ventral tegmental area(VTA)of the midbrain was observed by immunohistochem-istry.The levels of malonaldehyde,reduced glutathione and superoxide dismutase in the striatum of rats were measured by enzyme-linked immunosorbent assay.The levels of dopamine,5-hydroxytryptamine and noradrenaline in hippocampus of rats were detected by high performance liquid chromatography electrochemical method.The protein expressions of P38,p-P38,JNK,p-JNK,ERK and p-ERK in the substantia nigra of rats were detected by Western blot.RESULTS:After 4 weeks of RNSP administration,there was no significant different in body weight among the groups.Compared with normal group,the rotation frequency of the PD model group was increased significantly,the escape latency was significantly pro-longed,the number of table crossing times and the target-zone duration time were significantly decreased(P<0.05 or P<0.01).The number of TH positive neurons were significantly reduced in SNc and VTA;The levels of reduce glutathione and superoxide dismutase in the striatum were significantly decreased,and the level of malondialdehyde was significantly increased(P<0.05).The levels of dopamine,5-hydroxytryptamine and norepinephrine in the hippocampus were de-creased;The ratios of p-JNK/JNK and p-P38/P38 in the substantia nigra were significantly increased(P<0.05).Com-pared with PD model group,the rotation frequency of the RNSP groups was significantly decreased,the escape latency was significantly shortened,and the number of table crossing times and target-zone duration time were significantly increased(P<0.05).The number of TH-positive neurons,and the levels of reduced glutathione,superoxide dismutase,and dopa-mine were significantly increased,while the level of malondialdehyde and the ratios of p-JNK/JNK and p-P38/P38 were sig-nificantly decreased(P<0.05).The noradrenaline level in the medium and high dose groups and the 5-hydroxytryptamine level in the low and high dose groups were significantly increased,and the ratio of p-ERK/ERK in the medium and high dose groups were significantly decreased(P<0.05).CONCLUSION:RNSP can improve the cognitive ability of PD mod-el rats,and the mechanism may be related to inhibiting the P38/JNK/ERK signaling pathway,reducing the oxidative stress response in the midbrain,reducing the loss of DA neurons,and improving the monoamine neurotransmitters in the hippo-campus.关键词
藏药七十味珍珠丸/帕金森病/认知Key words
Tibetan medicine Ranasampel/Parkinson disease/cognition分类
医药卫生引用本文复制引用
崔海燕,闫清伟,李红艳,孙亚楠,郑博文,李腾,李稼霖,刘晓华..藏药七十味珍珠丸对帕金森病模型大鼠认知能力的影响[J].中国病理生理杂志,2024,40(4):610-618,9.基金项目
西藏自治区自然科学基金资助项目(No.ZRKX2020000075) （No.ZRKX2020000075）
西藏自治区自然科学基金重点项目(No.XZ202001ZR0087G) （No.XZ202001ZR0087G）
西藏自治区教育厅藏医药区域协同创新中心项目(No.2018XTCXOO3) （No.2018XTCXOO3）
西藏民族大学校内科研项目(No.22MDY08 （）
No.22MDY012) （）
陕西省自然科学基金资助项目(No.2021JM-477) （No.2021JM-477）
